摘要
目的探讨氨基葡萄糖对小鼠腹腔巨噬细胞(PMФ)免疫功能的影响。方法用壳聚糖制备氨基葡萄糖纯品,通过体外实验测定其对小鼠PMФ增殖、吞噬中性红、产生NO和IL-1能力的影响。结果氨基葡萄糖能够显著增强PMФ对中性红的吞噬能力,提高NO和IL-1的生成量,而对PMФ的增殖能力无明显影响。结论氨基葡萄糖能够活化PMФ,增强小鼠的免疫功能。
Objective To investigate the effect of glucosamine（GlcNH2） on immune function of murine peritoneal macrophages（PMФ）.Methods Pure GlcNH2 was prepared with chitosan,of which the effects on proliferation as well as abilities in phagocytosis of neutral red and producing NO and IL-1of PMФ were determined by in vitro tests.Results GlcNH2 enhanced the phagocytosis of neutral and increased red the production of NO and IL-1 by PMФ significantly,while showed no significant effect on the proliferation of PMФ.Conclusion GlcNH2 activated PMФ and enhanced the immune function of mice.
出处
《中国生物制品学杂志》
CAS
CSCD
2010年第9期946-948,952,共4页
Chinese Journal of Biologicals
基金
国家高技术研究发展计划(863计划)(2003AA625050)
