摘要
目的 研究大鼠缺血再灌注损伤和淋巴液引流后,Toll样受体4（TLR4）的内源性配体高迁移率族蛋白1（HMGB1）的表达以及远隔组织的损伤.方法 24只健康雄性SPF级SD大鼠被随机分为假手术组（Sham）、肠道缺血再灌注组（I/R）和肠道缺血再灌注＋淋巴液引流组（I/R＋引流）,每组8只.在肠道缺血再灌注损伤后检测远隔器官肺、肝和肾脏的损伤程度,肠道以及远隔器官肺、肝脏中TLR4内源性配体HMGBI的表达.结果 HE染色以及HMGB1免疫组织化学染色结果显示I/R组和I/R＋引流组损伤较Sham组重,L/R组细胞出现大量黄染,表明I/R损伤时HMGB1的表达增加,而I/R＋引流组的大鼠损伤显著轻于I/R组.Western blot检测显示I/R组的空肠、回肠、肝脏和肺组织中的HMGB1表达显著增加,其HMGB1/β-actin灰度值分别为0.3145±0.0549、1.7352±0.3280、1.4443±0.0926、3.1382±0.4202 引流淋巴液可以减轻损伤,其HMGB1表达均显著降低（P＜0.05）,HMGB1/β-actin灰度值分别为0.1745±0.0327、1.1083±0.2098、1.1862±0.1221、2.1095±0.1993.结论 大鼠肠道缺血再灌注损伤时,肠道和远隔组织的损伤与TLR4的内源性配体HMGB1表达增加呈正相关.淋巴引流能阻断＂肠-淋巴＂途径,减少肠道来源的HMGB1对肠道以及远隔组织的损伤.
Objective To investigate the expression of high mobility group box 1 （HMGBI） of TLR4 endogenous ligand and distant organ tissue injury after intestine ischemia/reperfusion and drainage of lymph fluid in rats. Methods Twenty-four Sprague-Dawley （SD） male rats （SPF grade） were evenly divided into 3 groups：Sham surgery group,intestine ischemia-reperfusion （I/R） group,and intestine ischemia-reperfusion with drainage of intestine lymph fluid （IR ＋ drainage） group.The injury of distant organs such as lungs,liver,kidney was evaluated The expression of high mobility group box 1 （HMGBI） of TLR4 endogenous ligand in intestine,lung and liver after the ischemia-reperfusion injury was measured by immunohistochemistry.Result HE stained sections,as well as HMGB1 immunohistochemistry results showed that the injury of ischemia/reperfusion （I/R） group and ischemia/reperfusion （I/R） ＋ drainage group were more severe than that in the sham group.A large number of cells stained in I/R group,indicating that HMGB1 expression increased.The injury in I/R ＋ drainage group was significantly less severe than I/R group.Western blot tests showed that the expression of HMGB1 in jejunum,ileum,liver,lung increased significantly in I/R group after L/R injury.Gray-scale values of HMGB1/β-actin were 0.3145 ± 0.0549、 1.7352 ± 0.3280、1.4443 ± 0.0926、3.1382 ± 0.4202.Lymph drainage significantly alleviated the damage,the expression of HMGB1 were significantly lower （P 〈0.05）.Gray-scale values of HMGB1/β-actin were 0.1745 ± 0.0327、 1.1083 ± 0.2098、 1.1862 ± 0.1221、2.1095 ± 0.1993. Conclusion Increased expression of HMGB1 of TLR4 endogenous ligand is associated with intestinal and distant tissue injury during intestinal ischemia-reperfusion injury.Drainage of lymph fluid can block the gutlymph pathway and thus reduce the source of HMGB1 from the intestinal as well as the injury of distant tissue.
出处
《中华普通外科杂志》
CSCD
北大核心
2010年第9期755-758,共4页
Chinese Journal of General Surgery
基金
基金项目：国家自然科学基金资助（30940069）
