摘要
目的:研究蛋白酶体抑制剂MG-132对心肌梗死大鼠心脏结构、功能的影响及其分子机制。方法:建立大鼠心肌梗死模型,干预组静脉注射MG-132(0.75mg/kg),对照组及假手术组注射生理盐水,观察各组大鼠心脏心肌结构、功能及心肌热休克蛋白70(Hsp70)、热休克蛋白70羧基端相互作用蛋白(CHIP)的变化。结果:与心梗对照组比较,MG-132干预组心肌坏死减少,左室舒张末压和血清脑钠肽水平减低(P<0.01),同时心肌组织Hsp70、CHIPmRNA和蛋白质表达水平显著升高(P<0.05)。结论:蛋白酶体抑制剂MG-132能够减轻心肌梗死、改善心功能,其机制与上调Hsp70、CHIP水平有关。
AIM：The study was designed to examine the effect of proteasome inhibitor MG-132 on cardiac structure and function in myocardial infarction rats, and to investigate its mechanism. METHODS： The myocardial infarction model was established by ligating rat＇s left anterior descending coronary artery. Proteasome inhibitor MG-132 was administered in a dose of 0.75 mg·kg-1·d-1 for 7 days. The myocardial pathological change, infraction volume, hemodynamics and brian natriuretic peptide were measured to evaluate the cardiac function; realtime PCR and Western blotting were applied to measure the mRNA and protein expressions of Hsp70 and CHIP. RESULTS： MG-132 decreased the levels of myocardial infraction volume, left ventricular end diastolic presssure and serum BNP （P0.05）. Meanwhile, MG-132 markedly elevated the mRNA and protein levels of Hsp70 and CHIP （P0.05）. CONCLUSION： MG-132 protects myocardium against acute myocardial ischemia injury by inducing CHIP and Hsp70 expression.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2010年第7期747-752,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
