摘要
水稻条纹病毒(rice stripe virus,RSV)NS3蛋白为病毒的沉默抑制子。利用酵母双杂交技术,从水稻cDNA文库中筛选出一个与RSV NS3蛋白互作的基因片段。推测该基因的功能是编码水稻3-磷酸甘油醛脱氢酶(glyceraldehyde-3-phosphate dehydrogenase,GAPDH)。双分子荧光互补实验进一步验证了NS3与GAPDH存在互作。瞬时表达实验表明,GAPDH与GFP基因融合蛋白在本氏烟表皮细胞细胞质中大量积累。此外,讨论了GAPDH蛋白在RSV侵染水稻过程中可能的功能。
NS3 protein of rice stripe virus （RSV） is an RNA silencing suppressor. Yeast two-hybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde-3-phosphate dehydrogenase （GAPDH）. Bimolecular fluorescence complementation （BiFC） also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDH-GFP fusion protein accumulated predominantly in cytoplasm of tobacco （Nicotiana benthamiana） through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2010年第5期493-496,共4页
Chinese Journal of Rice Science
基金
国家转基因生物新品种培育重大专项资助项目(2009ZX08009-044B)
国家自然科学基金资助项目(30770090)
农业部农业公益性行业科研专项资助项目(nyhyzx07-051)
关键词
水稻条纹病毒
蛋白功能
互作
酵母双杂交
双分子荧光互补
rice stripe virus
protein function
interaction
yeast two hybrid
bimolecular fluorescence complementation
