摘要
目的研究紫杉醇(抗癌药)对成纤维细胞基质金属蛋白酶-2(MMP-2)和α-平滑肌肌动蛋白(α-SM-actin)的影响。方法选取雄性SD大鼠,用贴壁法培养胸主动脉外膜成纤维细胞(AF),用RT-PCR方法检测α-SM-actin和MMP-2。结果成纤维细胞的MMP-2的表达量,紫杉醇组较对照组也明显降低(P<0.01);α-SM-actin的表达量,紫杉醇组较对照组明显降低(P<0.05),表明紫杉醇能抑制AF的MMP-2和α-SM-actin的表达,抑制AF的表型转化,从而抑制血管外膜的迁移。结论紫杉醇可防治冠状动脉介入术后再狭窄。
Objective To investigate the effect of paclitaxel on the ex- pression of matrix metalloproteinases - 2 （ MMP - 2 ） and α - smooth muscle aetin （ α- SM - actin） of the fibroblast. Methods Adventitial fibroblast （AF） were prepared from rat aortas using explant technique and cultured for passages, the expressions of MMP - 2 and α - SM - actin were determined by RT - PCR. Results The expression of MMP - 2 in the paclitaxel group is significantly lower than that in the control group （P 〈 O. 01 ）. The expression of α - SM - aetin in the paclitaxel group is signif- icantly lower than that in the control group（ P 〈 0. 01 ）. Paclitaxel can in- hibit the expression of MMP - 2 and α - SM - actin of the fibroblast, ef- fectively inhibiting the phenotype transformation of AF, thus inhibiting the multiplication and migration of vascular adventitia. Conclusion Paclita- xel can prevent restenosis after percutaneous coronary intervention.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2010年第9期665-667,共3页
The Chinese Journal of Clinical Pharmacology
基金
浙江省医药卫生科学研究基金资助项目(2007A198)
浙江省中医药科技计划基金资金项目(2007CA129)
关键词
紫杉醇
血管外膜
冠状动脉介入术后再狭窄
Α-平滑肌肌动蛋白
基质金属蛋白酶-2
paclitaxel
vascular adventitial
stenosis after percutaneous coronary intervention
α - smooth muscle actin
matrix metalloprotein- ases - 2
