摘要
目的寻找家族性２型糖尿病（２型ＤＭ）的遗传标志。方法采用Ｆｕｊｉｔａ等的红细胞膜钠锂逆转换活性（ＳＬＣＡ）测定方法，测定了家族性２型ＤＭ家系中无并发症的２２例家族性２型ＤＭ患者，２７例２型ＤＭ患者的非糖尿病一级亲属，４０例正常人的红细胞膜钠锂逆转换活性。结果家族性２型ＤＭ患者ＳＬＣＡ（０．３１０±０．１０１）ｍｍｏｌ·Ｌ－１·ＲＢＣ－１·ｈ－１，２型ＤＭ的一级亲属ＳＬＣＡ（０．２７４±０．１１２）ｍｍｏｌ·Ｌ－１·ＲＢＣ－１·ｈ－１，两者均显著高于正常对照组ＳＬＣＡ（０．１９１±０．０８３）ｍｍｏｌ·Ｌ－１·ＲＢＣ－１·ｈ－１（Ｐ＜０．０１）；家族性２型ＤＭ患者与其一级亲属之间ＳＬＣＡ无显著差异（Ｐ＞０．０５）。分别以ＳＬＣＡ、胰岛素敏感指数（ＩＳＩ）为应变量作逐步回归分析，发现ＩＳＩ和高密度脂蛋白组分２（ＨＤＬ２）与ＳＬＣＡ呈负相关（分别为Ｐ＜０．０１、Ｐ＜０．０５），ＩＳＩ、ＨＤＬ２对ＳＬＣＡ的影响程度为４２．５％，其中ＩＳＩ对ＳＬＣＡ的影响程度为３９．７％；并且ＳＬＣＡ参与ＩＳＩ的变易，ＳＬＣＡ对ＩＳＩ的影响程度为５５．７％。结论①家族性２型ＤＭ患者及其一级亲属ＳＬＣＡ明显增高；②红细胞膜ＳＬＣＡ?
Objective To searchfortheinheritedmarkersoffamilial type 2 diabetes mellitus. Methods Erythrocyte sodium lithium counter transport activity (SLCA) was measured according to the method described by Fujitain22familialtype2 DM patients (with 2 or more type 2 DM patients in the family), their 27 non diabetic first degree relatives, and 40 healthy control subjects. Results SLCA was significantly higher in familial type 2 DM patients (0.310±0.101)mmol·L -1 ·RBC -1 ·h -1 andtheirnon diabeticfirst degree relatives (0.274±0.112)mmol·L -1 ·RBC -1 ·h -1 than in healthy control subjects (0.191±0.083)mmol·L -1 ·RBC -1 ·h -1 (P<0.01) ,no significant difference being found between familial type 2 DM patients and their first degree relatives (P>0.05). A stepwise multiple regression analysis was conducted using SLCA and insulin sensitivity index (ISI) as the dependent variables respectively. It was found that SLCA correlated negatively with ISI andHDL 2 (P<0.01,P<0.05,respectively).Onthis model, ISI had the greatest influence (39.7%) on SLCA, and 42.5% of the variance in SLCA was explained by ISI and HDL 2. SLCA also had influence (55.7%) on ISI. Conclusion Erythrocyte SLCA increases significantly in familial type 2 DM patients and their first degree relatives and erythrocyte SLCA activity can be used as one of the indices for evaluation of insulin sensitivity as well as one of the inherited markers of familial type 2 DM.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1999年第3期142-145,共4页
Chinese Journal of Endocrinology and Metabolism
关键词
糖尿病
胰岛素抵抗
Ⅱ型
红细胞膜
SLCA
Familial type 2 diabetes mellitus
Insulin resistance
First degree relatives
