摘要
目的研究麻疯树叶提取物对人恶性黑色素瘤A375细胞增殖和凋亡的影响。方法用MTT法测定不同浓度麻疯树叶提取物对体外培养的人恶性黑色素瘤A375细胞增殖的抑制作用,采用倒置显微镜观察细胞形态学的改变,并用An-nexin-V-FIFT/PI双染色法检测细胞的凋亡。结果麻疯树叶提取物能抑制A375细胞增殖,诱导其凋亡,引起细胞形态学的改变,这些影响呈药物浓度剂量依赖性,并与药物作用时间正相关。结论麻疯树叶提取物在体外对人恶性黑色素瘤A375细胞的增殖具有显著的抑制作用,并能诱导其发生凋亡。
Objective To investigate the anticancer effects of extract from leaves of Jatropha curcas L.on the inhibition of cell proliferation and apoptosis induction in human melanoma cell line A375 in vitro.MethodsThe A375 cells was cultured with different concentration of extract from leaves of Jatropha curcas L.The cell proliferation inhibition of A375 cells were measured by methyl thiazolyl tetrazolium（MTT）colorimetric assay.The morphological changes of A375 cells were observed by the light microscope.The cell apoptosis ratio was assessed by Flow Cytometry with the Annexin-V-FIFT/PI affinity assay.ResultsExtract from Leaves of Jatropha curcas L.significantly inhibited the proliferation of A375 cells and it induced cell apoptosis and morphological changes in dose-and time-dependent manners.ConclusionExtract from leaves of Jatropha curcas L.can inhibit the cell proliferation and induce cell apoptosis of malignant melanoma cells.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2010年第9期2291-2293,共3页
Lishizhen Medicine and Materia Medica Research
基金
国家"十一五"科技支撑计划课题(No.2007BAD50B05)
教育部科技研究重大项目(No.307023)
关键词
麻疯树
黑色素瘤A375细胞
细胞增殖
细胞凋亡
Jatropha curcas L.
A375 cells
Malignant melanoma cells
Cell proliferation
Cell apoptosis
