摘要
目的:对犬外周血基质细胞(peripheral blood stromal cells,PBSCs)进行体外分离培养,研究其向成骨、成脂方向诱导分化的潜能。方法:取犬外周血,利用密度梯度离心法分离培养犬外周血基质细胞,免疫组化方法检测CD34和CD105表达情况;并用特定诱导液将分离的细胞向成骨细胞和脂肪细胞定向诱导分化。结果:该细胞表达CD105,不表达CD34;成骨诱导后,矿化结节茜素红染色阳性、OPN表达阳性;成脂诱导后,经油红O染色可见细胞内脂滴的积累。结论:利用密度梯度离心与贴壁培养相结合的方法可分离培养出外周血基质细胞,外周血基质细胞经诱导培养后具有多向分化潜能,有可能成为组织工程的种子细胞。
Objective：The peripheral blood stromal cells（PBSCs） of dogs were cultured in vitro to study their transdifferentiation to osteoblasts and adipocytes.Methods：PBSCs were isolated and developed with density gradient centrifugation method.The expressions of CD34 and CD105 on these cells were detected by immunohistochemistry.The cells were induced into osteoblasts and adipocytes with the inductive medium.Results：The cells were CD34（-） and CD105（＋）.After osteogenic induction,the cells were OPN（＋） and positively stained by alizarin Bordeaux.After adipogenic induction,the cells were full of lipid droplets and positively stained by oil red O.Conclusions：PBSCs can be successfully isolated and developed in vitro with density gradient centrifugation method and sticking wall method.The cells possess potential ability of orientation differentiation into osteoblasts and adipocytes.PBSCs are potential ideal candidates for seeding cells in bone tissue engineering.
出处
《中华老年口腔医学杂志》
2010年第3期133-136,共4页
Chinese Journal of Geriatric Dentistry
关键词
犬
细胞培养
外周血基质细胞
诱导分化
dog
cell culture
peripheral blood stromal cells
induced differentiation
