摘要
目的 用SD大鼠建立慢性牙周炎（CP）和慢性肾衰竭（CRF）动物模型,探讨两种疾病是否相关.方法 将40只健康雄性SD大鼠随机分为4组,每组10只：正常对照组、CP组、CRF组和CP＋CRF组.于8周末处死动物,检查记录牙周指标,检测血清中血肌酐（Scr）、尿素氮（BUN）、白细胞介素（IL）1β、肿瘤坏死因子（TNF）α的含量,观察牙周和肾脏组织病理学改变,进行统计学分析.结果 成功建立了CP和CRF动物模型.CP＋CRF组Scr（120.54±21.29）μmol/L,BUN（34.20±14.44）mmol/L;CRF组Scr（93.63±18.82）μmol/L.BUN（17.77±4.15）mmol/L,两组间差异有统计学意义（P〈0.05）.肾脏病理显示,CP组肾组织出现炎性改变.CP＋CRF组肾脏炎细胞浸润,PAS、Masson染色评分与CRF组差异有统计学意义.牙周病理显示,CP组、CP＋CRF组出现明显牙周炎,CP＋CRF组附着丧失（AL）（173.60±16.75）μm,CP组AL（124.00±23.87）μm,两组差异有统计学意义（P〈0.05）.CP组、CRF组、CP＋CRF组血清IL-1β、TNF-α水平显著高于正常对照组（P〈0.05）,CP＋CRF组IL-1β显著高于CP组、CRF组（P〈0.05）,CP＋CRF组TNF-α水平高于CP组、CRF组,且与CP组差异有统计学意义（P〈0.05）.2×2析因设计方差分析结果显示,CP与CRF对Scr、BUN、AL的数值具有交互作用（P〈0.05）,且两因素各自对上述指标的主效应显著（P〈0.05）;CP与CRF对IL-1β、TNF-α的数值不具有交互作用（P〉0.05）,但两因素各自对IL-1β、TNF-α的主效应显著（P〈0.05）.结论 本模型可在SD大鼠同时显现牙周炎和慢性肾衰竭,CP可加重肾衰竭,两者间有相关性,CP可能通过炎性机制加重肾脏的损害.
Objective To investigate the possible correlation between chronic periodontitis（CP）and chronic renal failure（CRF）by establishing chronic periodontitis and chronic renal failure model in SD rats. Methods Forty health male SD rats were divided into four groups： control group（A）, CP group（B）, CRF group（C）, CP accompany with CRF group（D）. Ten rats were sacrificed in every group at the end of week 8. The periodontal index, levels of serum Scr and BUN, the concentration of IL-1β and TNF-α were examined. The severity CP and CRF was quantified by histopathology. The date was statistically analyzed. Results Animal models were established successfully. Scr and BUN in group D, BUN were higher than that in group C[Scr（120.54±21.29）junol/L vs（93.63±18.82）u,mol/L, BUN（34.20±14.44）mmol/L vs（17.77±4.15）mmol/L, P〈0.05]. The kidney change of inflammation was observed in group B, the grade of PAS and Masson in group C and D were higher than that in group A（P〈0.01）, and that in group D was higher than group C（P〈0.05）. Obvious inflammation of periodontal tissue was observed in group B and D. Attachment loss level（AL）in group D was higher than that in group B[（173.60± 16.75）μm vs（124.00±23.87）μm, P〈0.05]. The level of IL-lβ and TNF-α in group B and C and D were higher than that in group A（P〈0.05）, and IL-lβ in group D was higher than that in group B and C（P〈0.05）, TNF-a in group D was higher than that in group B（P〈0.05）. 2×2 factorial design revealed that there were interactions between CP and CRF on the numerus of Scr and BUN and AL P〈0.05）, and the influence of each factor on that was significant（P〈0.05）, no interactions were noted between CP and CRF on IL-1β and TNF-α（P〉0.05）, but the influence of each factor on that was significant（P〈0.05）. Conclusions The SD rat models can appear chronic periodontitis and chronic renal failure at the same time. There is correlation between chronic periodontitis and chronic
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2010年第8期619-623,共5页
Chinese Journal of Nephrology
