摘要
根据GenBank上猪GAPDH基因的序列设计并合成一对引物,采用SYBR GreenⅠ染料建立了Real-time PCR方法。以阳性重组质粒为标准品,建立了标准曲线,并进行了融解曲线分析。结果表明,所建立的方法具有快速、高通量、线性范围广以及重复性强等特点。研究结果为GAPDH作为内参基因用于猪相关基因定量表达分析奠定了基础。
According to the swine GAPDH gene sequences available in GenBank,a pair of primers was designed for establishing a SY BR Green I quantitative real-time PCR method for swine GAPDH gene of swine.To establish the standard curve,the plasmids served as a standard.The analysis of melting curve was also carried out.The results showed that the real-time PCR method established in the study had advantages of rapid,high-throughput,wide linear range and good reproducibility.The results of this study could provide basis for GAPDH used as a reference gene to analyze the swine gene quantitatively.
出处
《西南农业学报》
CSCD
北大核心
2010年第4期1282-1285,共4页
Southwest China Journal of Agricultural Sciences
