摘要
目的探讨N-cadherin和相关因子β-catenin mRNA在人脑干和幕上胶质瘤中的表达。方法采用RT-PCR法检测N-cadherin和β-catenin mRNA在18例脑干胶质瘤和18例幕上胶质瘤中的表达。结果 N-cadherin mRNA在脑干和幕上胶质瘤中的表达无显著性差异(P>0.05),β-catenin mRNA在脑干胶质瘤中的表达高于幕上胶质瘤(t=2.255,P<0.05);N-cadherin mRNA在脑干胶质瘤中随着肿瘤级别的升高而增高(t=2.711,P<0.05),在幕上胶质瘤各级别表达无显著性差异。β-catenin mRNA在不同级别脑干和幕上胶质瘤中的表达均无显著性差异(P>0.05);N-cadherin和β-catenin mRNA在脑干和幕上胶质瘤中的表达均呈正相关(r=0.480,r=0.809,均P<0.05)。结论 N-cadherin和β-catenin的过度表达在人脑干胶质瘤的侵袭及恶性进展中起着重要作用。
Objective To explore the expression of N-cadherin and β-catenin mRNA in human brainstem and supratentorial gliomas.MethodsN-cadherin and β-catenin mRNA expression in 18 cases of brainstem gliomas and 18 cases of supratentorial gliomas tissues were detected with PT-PCR.Resultsβ-catenin mRNA expression was more in human brainstem gliomas than in supratentorial gliomas（t=2.255,P〈0.05）,but was not significantly different of N-cadherin mRNA（P〈0.05）.The expression of N-cadherin mRNA in human brainstem gliomas of grades Ⅰ~Ⅱ were less than those in human gliomas of grades Ⅲ~Ⅳ（t=2.711,P〈0.05）,but was not of β-catenin mRNA（P〈0.05）.N-cadherin mRNA expression was positively correlated with the β-catenin mRNA expression in either brainstem gliomas or supratentorial gliomas（r=0.480,r=0.809 respectively,P〈0.05）.ConclusionThe over expressions of N-cadherin and β-catenin may play an important role in the invasion and malignant progress of human brainstem gliomas.
出处
《中国康复理论与实践》
CSCD
2010年第9期856-858,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
国家自然科学基金(30772237
3090049)
