摘要
目的:建立养精种玉汤(白芍、山茱萸、当归和熟地黄)正丁醇部位的指纹图谱。方法:采用HPLC法,色谱柱为Gemini C18柱(250mm×4.6mm,粒度5μm);流动相为乙腈-0.1%磷酸(非线性梯度洗脱);检测波为225nm;柱温为25℃;流速为1mL/min。结果:指纹图谱中共有19个特征峰,其中白芍有7个特征峰,山茱萸有8个特征峰,当归有5个特征峰,熟地黄有2个特征峰。结论:建立的指纹图谱适用于养精种玉汤及其制剂的质量控制。
AIM： To set up the fingerprint of n-butanol extract of Yangjing Zhongyu Decoction（Radix paeoniae alba,Fructus corni,Radix angelicae sinensis,and Radix rehmanniae praeparate）. METHODS： HPLC analysis was adopted in the fingerprint,and the chromatographic conditions were as follows： the column used was Gemini C18（250 mm × 4. 6 mm,5 μm granularity）; The mobile phase was acetonitrile-0. 1% phosphorous acid （non-line-ar grade elution）; The detection wavelength was set up at 225 nm; The temperature of the column was at 25 ℃; The elution rate was 1 mL/min. RESULTS： There were mainly 19 characteristic peaks in the fingerprints. Among these peaks,7 peaks belonged to Radix Paeoniae Alba,8 peaks belonged to Fructus Corni,5 speaks belonged to Radix Angelicae Sinensis,and 2 peaks belonged to Radix Rehmanniae Praeparata. CONCLUSION： The finger-print can be used to control the quality of Yangjing Zhongyu Decoction and its preparations.
出处
《中成药》
CAS
CSCD
北大核心
2010年第9期1461-1464,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金(30701119)
中山市科技计划项目(2009H019)
