摘要
目的应用傅里叶变换红外(FTIR)光谱技术分析大鼠死后脾脏组织随死亡时间增加的化学变化过程,为死亡时间推断研究提供新的途径与研究数据。方法大鼠断颈处死后,在30℃、20℃及4℃环境中,不同死亡时间点提取大鼠脾脏组织,并运用FTIR光谱仪测定不同化学基团随死亡时间的变化。结果随着死亡时间的推移,大鼠脾脏组织FTIR光谱的主要吸收峰峰位没有明显变化,而其吸收峰强度有明显差异:(1)1080cm-1和1238cm-1被指认核酸谱带吸收峰的峰强呈下降趋势;(2)1541cm-1被指认酰胺Ⅱ吸收峰的峰强呈上升变化;(3)1396cm-1被指认脂肪酸吸收峰的峰强呈上升变化;(4)指认为C-H结构振动的2852、2871、2923、2958cm-1吸收峰的峰强呈现上升趋势。结论 FTIR光谱分析技术有望成为法医死亡时间推断的有效方法。
Objective To explore chemical changes in the rat spleen postmortem using FTIR spectroscopy and to provide a new method and research data for estimation of postmortem interval.Methods Rats were sacrificed by cervical dislocation and the cadavers were kept at 30℃, 22℃-26℃ and 4℃ in a controlled environment chamber.The spleens were subsamples at different postmortem time points.All spleen tissues were measured using FTIR spectrometer.Results The absorption peak position showed no changes after death, but absorption intensity displayed obvious changes：（1）the absorption intensity assigned to nucleic acid （1080 cm-1 and 1238 cm-1） showed decreasing tendency; （2）the absorption intensity assigned to amide Ⅱ（1541 cm-1） showed increasing tendency; （3）the absorption intensity assigned to fatty acid （1396 cm-1） increased.（4）the absorption intensity assigned to C-H vibration （2852, 2871, 2923, 2958cm-1） showed increasing tendency.Conclusion FTIR spectroscopy can be an effective method for the estimation of postmortem interval.
出处
《中国司法鉴定》
北大核心
2010年第5期38-42,共5页
Chinese Journal of Forensic Sciences
基金
国家自然科学基金资助项目(30471935)
上海市自然科学基金资助项目(09ZR1432900)
关键词
傅立叶变换
红外光谱
死亡时间
脾脏
Fourier transform
infrared spectrometry
postmortem interval
spleen
