摘要
最新研究显示Th17细胞可能参与了非病毒特异性肝损伤的调控，然而至今尚无研究报道其是否参与了乙型肝炎后肝硬化的肝损伤。目的：探讨Th17细胞及其细胞因子与乙型肝炎后肝硬化肝组织炎症活动度的关系。方法：收集12例肝组织发生亚大块及以上坏死、12例肝组织炎症活动度为G2~G4的乙型肝炎后肝硬化患者以及11例健康人的肝组织和外周血标本，以免疫组化方法检测肝组织IL-17的表达和分布，实时荧光定量PCR检测肝组织Th17型细胞因子IL-17 mRNA和Th17细胞特异性转录因子RORγt mRNA的表达，ELISA方法检测血清IL-17水平。结果：亚大块坏死组肝组织IL-17阳性细胞浸润区域较G2~G4炎症组广泛，健康对照组仅有少量IL-17阳性细胞浸润。亚大块坏死组肝组织IL-17、RORγt mRNA表达和血清IL-17水平均明显高于G2~G4炎症组（P=0.0226, P=0.0531, P=0.0171），G2~G4炎症组又高于健康对照组（P=0.0289, P=0.0005, P=0.0160）。肝内IL-17 mRNA表达和血清IL-17水平与肝组织炎症活动度呈正相关（rs=0.686, P〈0.0001; rs=0.767, P〈0.0001）。结论：肝内和血清Th17型细胞因子IL-17水平与乙型肝炎后肝硬化肝组织炎症的严重程度呈正相关，Th17细胞及其细胞因子参与了乙型肝炎后肝硬化的肝损伤。
Recently, some studies demonstrated that Th17 cells may be involved in the modulation of non-viral specific liver injury, however, the role of Th17 cells in liver injury of post-hepatitis B cirrhosis remains unknown. Aims： To elucidate the relationship between Th17 cells, Th17 cytokine and the inflammatory activity of liver tissue in post-hepatitis B cirrhosis. Methods： Twelve patients with HBV-associated acute-on-chronic liver failure （ACLF group）, 12 patients with G2-G4 grade HBV-associated liver cirrhosis （LC group） and 11 healthy controls （HC group） were enrolled. Liver tissue and peripheral blood samples were collected. Immunohistochemical method was used to measure the expression and distribution of IL-17 in liver tissue, real-time fluorescence quantitative PCR was used to determine the expressions of Th17 cytokine IL-17 mRNA and Th17 cell specific transcription factor RORγt mRNA in liver tissue, and ELISA was used to measure the level of serum IL-17. Results： Area infiltrated with IL-17 positive cells in ACLF group was significantly greater than that in LC group, while only few IL-17 positive cell infiltration was seen in HC group. Hepatic IL-17, RORγt mRNA expressions and serum IL-17 level in ACLF group were significantly higher than those in LC group （P=0.0226, P=0.0531, P=0.0171）, and those in LC group were significantly higher than those in HC group （P=0.0289, P=0.0005, P=0.0160）. Hepatic IL-17 mRNA expression and serum IL-17 level were positively correlated with the inflammatory activity of liver tissue （ rs=0.686, P〈0.0001; rs=0.767, P〈0.0001）. Conclusions： Levels of Th17 cytokine IL-17 in liver tissue and serum are positively correlated with the severity of inflammation of liver tissue in post-hepatitis B cirrhosis, and Th17 cells and Th17 cytokine are involved in the liver injury of post-hepatitis B cirrhosis.
出处
《胃肠病学》
2010年第8期456-461,共6页
Chinese Journal of Gastroenterology
基金
“十一五”863计划重大课题(2006AA02A411)
上海市卫生局科技发展基金项目(044050)资助
