摘要
本文提出一种测定FeMo-co催化活力的反应体系,用此反应体系,在测定FeMo-co催化活力的过程中,FeMo-co与变种UW45抽提液的重组活性始终保持不变。讨论了水含量、还原剂对FeMo-co催化活力和重组活力的影响。
A water-free reaction system for determining catalytic activity of Fe-Mo-co has been developed. Comparison between reconstitutive activity and catalytic activity of FeMo-co in water and water-free systems has been made. The results show that free FeMo-co requires hydrophilic environment for its catalytic activity, while the FeMo-co in reconstituted form requires hydrophobic environment for its reconstitution activity. The effects of water content and reductant concentration in the reaction systems on catalytic activity of FeMo-co were discussed.
关键词
固氮酶
铁钼辅因子
重组活力
催化
FeMo cofactor, Nitrogenase, Reconstitutive activity assay, Catalytic activity determination
