摘要
目的 探讨苦碟子注射液（KDI）对Lewis肺癌体内外的抑制作用及其机制.方法 12.5～400.0g/L KDI分别作用Lewis肺癌细胞后,噻唑蓝（MTT）比色法检测KDI体外对Lewis肺癌细胞的抑制作用.用C57BL/6小鼠Lewis肺癌模型,观察不同浓度KDI的抗癌作用,流式细胞仪检测肿瘤细胞凋亡及细胞周期.结果 KDI能显著抑制Lewis肺癌细胞的增殖,并呈剂量效应.低、中、高剂量组对Lewis肺癌的抑瘤率分别为21.64%、36.43%、46.68%,均明显高于生理盐水组（P＜0.01）;流式细胞仪检测发现,KDI低、中、高剂量组G0/G1期细胞数分别为（68.10±3.43）%、（80.06±5.48）%和（78.36±6.68）%,S期细胞比例分别为（30.98±1.99）%、（17.48±6.36）%和（17.05±6.35）%,KDI能够使Lewis肺癌细胞阻滞于G0/G1,凋亡率分别为8.96%、17.40%、10.34%,均显著高于生理盐水组（P＜0.05）.结论 在体内外,KDI对Lewis肺癌均有明显的抑制作用,可能与其诱导细胞凋亡并使细胞周期阻滞于G0/G1期有关.
Objective To observe the inhibition effects to Lewis lung cancer of kudiezi injection （KDI） in vitro and in vivo. Methods After administration of 12.5-400. 0 g/L KDI for 24-72 h, the MTT method was used to investigate the inhibitory effect of KDI on Lewis lung cancer cells. Cell apoptosis and cell cycle arrest were investigated by flow cytometry （FCM）. Results KDI significantly inhibit the growth of Lewis lung cancer cells in dose-dependent manners. FCM revealed that after treatment with different dose of KDI （5,10,20 g/kg）for 14 d,the number of G0/G1 stage cell was （68. 10 ±3.43）%, （80.06 ±5.48）%,（78.36 ± 6. 68） %, and that of S stage cells was （ 30. 98 ± 1.99 ） %, （ 17. 48 ± 6. 36） %, （ 17. 05 ± 6. 35 ） %respectively, and that of apoptotic rate was 8. 96%, 17.40%, 10. 34% respectively. Conclusion KDI can inhibit Lewis lung cancer cells in vitro and in vivo,which was probably contributed to induce apoptosis and G0/G1 cell cycle arrest in Lewis lung cancer cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2010年第9期1208-1210,共3页
Chinese Journal of Experimental Surgery
基金
湖北省自然科学基金资助项目（2009CBD054）
武汉市科技攻关资助项目（20056101017）
