摘要
目的:研究四逆散冻干粉改善果蝇睡眠作用的量-效与时-效关系。方法:选取野生型Canton S品系黑腹果蝇为试验对象,利用自动红外果蝇活动监测系统,以果蝇24 h总睡眠时间为观察指标,分别进行四逆散冻干粉改善睡眠作用的量-效与时-效关系的研究。结果:雌雄果蝇的24 h总睡眠时间随着给药剂量的增加而增加,具有一定的剂量依赖关系,雌果蝇的最大有效剂量是4%,而雄果蝇的最大有效剂量为2%,雌果蝇连续给药4 d果蝇24 h总睡眠时间最长,而雄果蝇连续给药7 d,其24 h总睡眠时间最长。结论:四逆散冻干粉能促进果蝇的睡眠,但对雌、雄果蝇睡眠的影响程度不同。雌果蝇给药高浓度短时间效果较好,雄果蝇给药低浓度长时间效果好。
Objective： This paper aims to identify the relation of the dose-effect and time-effect of freezingdried powder of Sinisan for sleep improvement.Method： We selected the 7-day-old wild-type Canton S Drosophila melanogaster,and used an Infrared Drosophila Activity Monitor System（DAMS;Trikinetics Inc.USA） to record sleep state of them.We observed and analyzed the recording data for continuous 24 hr.Then we studied the doseeffect and time-effect of Freezing dried powder of Sinisan in Drosophila sleep improvement.Result： 24 hour total sleep time of Drosophila increased as dose increased,it had a definite dose-effect.4% drug substratum for dose,4 days for delivery time is best for female,and 2% drug substratum for dose,7 days for delivery time is best for male.Conclusion： Freezing dried powder of Sinisan can promote the Drosophila sleep,but there are different influence degree in male and female Drosophila.Administration of high concentrations and short-term is best fo female,and low concentrations and long-term is best for male.
出处
《中国实验方剂学杂志》
CAS
北大核心
2010年第12期109-111,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30672634)
