摘要
从广西金芽、四川阿西所属的三个金矿区采集了土壤样品55份，采用选择性培养基从10^(-2)稀释度的平皿中分离到阳性菌739株，从中挑取11株，其分出率为1．5％，并对分离株进行了光镜和电镜形态学观察，其大小为1～1．4×2．5～3．5μm；芽孢椭圆形．中生，没有孢囊和伴孢晶体，并对11株分离株的生理生化特性进行了测定，结果表明，除C_（17）分离株V、P反应阴性有待进一步分析外，其余10株都与标准株完全相同，因此，应归属芽孢杆菌属蜡状芽孢杆菌（Bacilluscereus）．
The isolation and identification of Bacillus cereus from soil samples ofgold deposit in Guangxi and Sichuan Provinces were reported. Fiftyfive soil sampleswere collected from 3 deposits. A total of 739 Positive colonies were isolated byEYA selective medium and 11 isolates were picked out from the colonies, the rateof isolation is 1. 5%. The morphology of cells and spores of these strains was inves-tigated under light and electron-microscope. The cells are Grampositive rods, 1. 0to 1. 4 pm by 2. 5 to 3. 5 pm, The spores are elliptical, central and without sporani-um and parasporal crystal. The physiological and biochemical characteristics of 11strains were examined. The results indicated that 10 of them should be identified asBacillus cereus
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
1999年第2期271-277,共7页
Journal of Central China Normal University：Natural Sciences
基金
地矿部生物成矿作用重点学科生长点基金
湖北省科委重点项目
关键词
金矿区
土壤样品
蜡状芽孢杆菌
分离
鉴定
gold deposit
soil samples
Bacillus cereus
isolation
identification
