摘要
目的研究Hes1、Hes5基因沉默对神经胶质瘤细胞U251增殖的影响。方法构建Hes1-shRNA和Hes5-shRNA慢病毒表达载体,分别干扰U251细胞Hes1、Hes5基因表达,用MTT、克隆形成实验和流式细胞术检测细胞增殖及克隆形成能力。结果沉默Hes1或Hes5基因均能明显抑制U251细胞增殖及集落形成。结论Hes1、Hes5直接参与了U251细胞的增殖调控,可作为胶质瘤基因治疗的潜在作用靶点。二者对U251细胞增殖的影响无显著性差异。
Objective To study the effect of Hes1 and Hes5 gene silencing on proliferation of U251 cells.MethodsLentiviral vectors expressing shRNA targeting human Hes1 and Hes5 gene were constructed to interfere Hes1 and Hes5 gene expression in U251 cells.MTT assay,plate clone formation assay and flow cytometry were performed to assay the proliferation and colony formation ability of U251 cells.Results Proliferation and colony formation of U251 cells can be effectively inhibited by Hes1 or Hes5 gene silencing.Conclusion Hes1 and Hes5 are directly involved in proliferation regulation of U251 cells,and can be regarded as potential gene therapeutic targets of glioma.There are no difference between them in effecting proliferation of U251 cells.
出处
《基础医学与临床》
CSCD
北大核心
2010年第9期976-980,共5页
Basic and Clinical Medicine
基金
福建省自然科学基金(C0810017)
福建医科大学研究基金(JS06008)
