摘要
目的:探讨肝再生过程中线粒体通透性转换的作用.方法:将SD大鼠105只随机分成3个实验组:肝再生(PH)组、环孢菌素A(CsA)处理组和对照(SH)组,每组取7个时间点;PH组和CsA组大鼠经70%肝切除,CsA组用CsA进行灌胃.用透射电镜观察线粒体超微结构的变化,并检测肝组织匀浆内丙二醛(MDA)的变化.结果:透射电镜显示在PH后24h时线粒体发生重度肿胀,线粒体明显增大变圆,基质变淡甚至完全空泡化,嵴明显缩短甚至消失;72时中度肿胀,6h、120h时轻度肿胀,内质网也有类似的变化;CsA组在0h、3h和6h时轻度肿胀,24h中度肿胀,72h和168h时则明显收缩.肝组织中MDA在PH后3h时轻度升高,到24h时达到最高,之后逐渐下降,到120h时恢复正常.CsA组MDA的变化规律与PH组的基本一致,但在(0-120h)的各个时间点自由基含量明显高于PH组.结论:肝再生过程中,线粒体的超微结构变化和肝组织MDA的变化可能与线粒体通透性转换有关,尤其在24h时变化最显著,这种变化与肝再生的启动密切相关.
AIM：To investigate the role of mitochondria permeability transition（MPT） in liver regeneration.METHODS：One hundred and five male Sprague-Dawley rats were randomly divided into three groups：partial hepatectomy（PH） group,cyclosporin A（CsA） group and sham-operated（SH） group.Animals in the PH and CsA groups underwent 2/3 partial hepatectomy.Animals in the CsA group were administered CsA before the surgery.The animals in each group were further divided into seven sub-groups.Ultrastructural morphology of mitochondria in remnant liver after PH was determined by electron microscopy.The content of malondialdehyde（MDA） in liver tissue was also measured.RESULTS：Remarkable changes were observed in the morphology and ultrastructure of the liver mitochondria at 24 h after PH,including conspicuous swelling,increased membrane permeability,reduced number of cristae,and matrix vacuolation.At 72 h,moderate mitochondrial swelling was observed,while,at other time points,mild mitochondrial swelling was seen.Mitochondrial permeability increased at 0,3 and 6 h in the CsA group,but decreased at 24 and 72 h when compared with the PH group.Similar changes in endoplasmic reticulum were also noted.The content of MDA increased at 3 h after PH,peaked at 24 h,and then decreased and returned to normal level at 120 h.The contents of MDA at all time points in the CsA group were significantly higher than those in the PH group.CONCLUSION：The changes in mitochondrial ultrastructure and MDA level are closely associated with MPT during live regeneration in rats,especially prominent at 24 h after PH.The changes in mitochondrial ultrastructure and MDA level in liver tissue is possibly related to the initiation of liver regeneration after PH.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第22期2302-2307,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30570837~~
关键词
肝再生
超微结构
自由基
Liver regeneration
Mitochondrial ultrastructure
Malondialdehyde
