摘要
目的:建立固相萃取反相高效液相色谱法测定芪黄胶囊中大黄素的含量方法。方法:采用ODS-C18小柱处理样品,ZorbaxSB-C18柱(4.6mm×250mm,5um),流动相:甲醇:0.1%磷酸溶液(90:10),流速:1.0mL·min^(-1),检测波长:254nm。结果:线性范围1.0-5.0μg(r=0.9999),平均回收率100.31%,RSD=2.36%。结论:该法简便、快速、准确。
Objective ： To establish a method for the determination of Emodin in Qihuang capsules using a solid - phase extraction and reversed - phase HPLC. Methods ： The sample was treated with ODS- C18 solid- phase extraction colunm, Zorbax SB - C18 column（4.6mm × 250mm,5um） was used, MeOH - 0.1% H3 PO4 （90： 10）were served as mobile phase with 1.0mL·min^-1 flow rate. The detection wavelength was 254nm. Results： Emodin concentration presented a good linear range of 1.0- 5.0μg（r = 0.9999）, the average recovery was 100.31%, RSD = 2.36 %. Conclusion： This method is rapid, simple and accurate.
出处
《内蒙古中医药》
2010年第15期110-111,共2页
Inner Mongolia Journal of Traditional Chinese Medicine
