摘要
目的:建立高效液相色谱-库仑阵列电化学法测定尿液中的神经内分泌瘤标志香草扁桃酸、高香草酸和5-羟吲哚乙酸,以及连用紫外检测法同时测定肌酐浓度的方法。方法:尿样经稀释离心后直接进样,采用50mmol/L磷酸二氢钠水溶液中分别含1%和40%的乙腈作为两相梯度分离,柱温35℃,主通道检测电压为160、260和360mV,紫外检测波长为210nm。结果:在浓度为0.1～1.5mg/L范围内,神经内分泌瘤标志的线性关系良好(r>0.9992),肌酐在30～450mg/L范围内线性关系良好(r=1.0000)。5-羟吲哚乙酸、香草扁桃酸、高香草酸和肌酐的平均回收率依次为96.9%、98.5%、101.2%和102.6%。四种物质的组内变异系数均<4%,组间变异系数均<6%。成人神经内分泌瘤患者的特征标志的值>正常对照值。结论:研究建立了快速准确同时检测随机尿样高香草酸、香草扁桃酸、5-羟吲哚乙酸和肌酐的方法,适用于临床神经内分泌瘤的诊断及其他神经精神等疾病的研究。
OBJECTIVE：To develop a HPLC-ECD method on simultaneous determination neuroendocrine tumor markers：5-hydroxyindoleacetic acid （5-HIAA）,vanillylmandelic acid （VMA）,homovanillic acid （HVA）,and determination Cr using UV detector.METHODS：Samples were simply diluted and filtered before separation by binary gradient reversed phase chromatography.The mobile phase consisted of 50 mmol/L sodium dihydrogen phosphate with 1% and 40% acetonitrile as binary phases.The column temperature was 35 ℃.The dominant detection voltage was 160,260 and 360 mV.RESULTS：The calibration curve was linear in the range of 0.1～1.5 mg/L（r〉0.999 2） for the neuroendocrine tumor markers and in the range of 30～450 mg/L（r=1.000 0） for Cr.The methodological recovery of 5HIAA,VMA,HVA,and Cr was 96.9%,98.5%,101.2% and 102.6%,respectively.The intra-assay CV were less than 4% and inter-assay CV were 1ess than 6%.The values of major neuroendocrine tumor markers in adult patients were more than normal ranges.CONCLUSION：This HPLC method is accurate,sensitive,simple,and suitable for diagnosis of neuroendocrine tumor or other study such as neuropsychiatric disorders.
出处
《中华肿瘤防治杂志》
CAS
2010年第14期1092-1095,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(30671156)
北京自然科学基金(5072014
7042017)
北京优秀人才培养(2008D0303200106)
关键词
神经内分泌瘤/诊断
色谱法
肿瘤标志
neuroendocrine tumor/diagnosis
chromatograpy
tumor marker
