摘要
从新疆昌吉州吉木萨尔野马饲养繁殖中心送检的野马病料中分离到一株病毒，并对其进行了全面鉴定．该病毒株在ＢＨＫ－２１细胞上连传６代出现典型细胞病变，用适应ＢＨＫ－２１细胞的病毒接种鸡胚成纤维细胞、乳豚鼠肾原代细胞、豚鼠睾丸细胞均出现程度不同的细胞病变．在电镜下可见到典型的马鼻肺炎病毒粒子（疱疹病毒样颗粒）．病毒对５－碘脱氧尿核苷、氯仿、乙醚等敏感．在ｐＨ３下失活，５６℃３０ｍｉｎ灭活．病毒能被马鼻肺炎病毒标准阳性血清所中和．病毒接种乳豚鼠出现典型致死性肝炎．结果表明所分离病毒为马鼻肺炎病毒。
We had isolated a virus strain from the sample of Przewals kii Horse at Xinjiang Przewalskii Horse Breeding Centre in Jimsar County,Changji ,Xijiang Uygor Autonomous Region.The virus had been identified.It was continuall y inoculated 6 generations onto hamster kindey cell (BHK-21) and induced typical cytopathogenic effect (CPE).Culturing the virus on chicken embryonic fibroblast cell,sucking geineapig kindey and testicle cell,the CPE could be seen but there is different.The typical equine rhionopneumonitis virus particles (Like Herpesv irus particles) could be found under electromicroscope.The isolated virus was se nsitive to 5-iod-2-dexoy uridine,Chloroform,ether etc.It loses activity in pH3 a nd was inactivated for 30 minutes at 56℃.The virus could be neutralized by the standard positive serum to equine rhinopneumonitis virus.The sucking guineapigs were injected with the isolates and the typical lethal hepatitis could be observ ed.All of the evidences indicated that the isolated virus was equine rhinopneumo nitis virus (ERV),and it was named PH93-1 strain of equine rhinopneumonitis viru s.
出处
《云南大学学报（自然科学版）》
CAS
CSCD
1999年第3期182-185,共4页
Journal of Yunnan University(Natural Sciences Edition)
关键词
野马
马鼻肺炎病毒
分离
鉴定
przewalskii horse
equine rhinopneumonitis virus
isolat ion and identification
