摘要
分别以麝香百合（Longiflorum,L）White Fox、亚洲百合（Asiatic,A）Conneticut King、东方百合（Oriental,O）Sorbonne及喇叭百合（Trumpet,T）Royal Goden为亲本,用切割柱头法进行杂交,采用离体培养技术对杂种幼胚进行抢救,并运用基因组原位杂交技术对所得杂种进行鉴定.结果显示,在所选的6个杂交组合中,切割柱头授粉能不同程度地克服除LL×TT之外的5个杂交组合的受精前障碍;胚、胚乳及胚珠3种外植体的离体培养萌发率分别为100%、21.5%和1%,所得植株占所得杂种的比例分别为9.6%、89.9%和0.5%;基因组原位杂交分析显示,所获植株均为真杂种.结果证明,胚、胚乳及胚珠培养是从幼胚获得百合杂种植株的高效途径,且能有效防止伪杂种混杂,胚乳培养是获得百合杂种一代的主要方式.
Interspecific hybridization was undertaken between lily cultivars from different hybrid groups,viz.＇White Fox＇from Longiflorum,＇Conneticut King＇from Asiatic,＇Sorbonne＇from Oriental and ＇Royal Goden＇from Trumpet,cut-style pollination method and embryo rescue technique were used to overcom hybridization barriers,and the hybrids were identified by genomic in situ hybridization（GISH）.The results showed that cut-style pollination significantly increased the crossing efficiency except the LL×TT combination,the efficiency of embryo,embryo sac and ovule culture were 100%,22% and 1% respectively,and 9.6%,89.9% and 0.5% of the progeny were finally obtained.Genomic in situ hybridization indicated that all of them were the real hybrids.It can be concluded that embryo,embryo sac and ovule culture are efficient methods for overcoming interspecific hybridization barriers,without false hybrid mixture.Embryo sac culture is the predominant way to obtain hybrids.
出处
《西北植物学报》
CAS
CSCD
北大核心
2010年第8期1573-1578,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家林业局项目(2006-73)
关键词
百合
系间杂交
胚乳培养
胚培养
胚珠培养
基因组原位杂交(GISH)
lily（Lilium）
interspecific hybridization
embryo sac culture
embryo culture
ovule culture
genomic in situ hybridization（GISH）
