摘要
目的评价BACTEC树脂需氧瓶和树脂厌氧瓶对血液中病原菌的检测能力。方法回顾性分析1241例BACTEC树脂需氧瓶、树脂厌氧瓶配对血液培养结果,比较分离病菌的种类及不同培养瓶报阳时间。结果 1 241份血液标本有138份报阳,分离病原菌141株(3份标本为复数菌感染)。需氧瓶和厌氧瓶分离病原菌126株和122株,107株病原菌可同时在两种血培养瓶生长,另有19株和15株分别只能在需氧瓶或厌氧瓶中生长。50%的肠杆菌和60%的阳性球菌的在厌氧瓶中生长迅速而先于需氧瓶报阳。结论厌氧树脂瓶检测菌血症有一定优势,联合使用需氧、厌氧瓶进行血培养能更有效、更快速诊断菌血症。
Objective To evaluate the performance of BACTEC Plus Aerobic/F and Plus Anaerobic/F medium for recovery of pathogenic microorganisms from adult patients. Methods All blood cultures were performed by the BACTEC 9120 System with paired Plus Aerobic/F and Plus Anaerobic/F Blood Culture bottles according to the protocol. The overall recovery of organisms and time to detect were analyzed retrospectively. Results 141 clinically important isolates were recovered from 138（250 bottles）patients with positive blood culture,of these isolates, 126 of 141 isolates were recovered from Plus Aerobic/F and 122 of 141 isolates were recovered from Plus Anaerobic/F bottles. On the other band, 107 were recovered from both pairs of bottles, with 19 recovered from only Plus Aerobie/F bottles and 15 recovered from only Plus Anaerobic/F. Conclusion Plus Anaerobie/F bottles enhanced recovery of pathogenic microorganisms from patients . the combination of Plus Aerobic/F and Plus Anaerobic/F bottles showed better recovery than either of Plus Aerobie/F or plus Anaerobic/F bottles.
出处
《国际检验医学杂志》
CAS
2010年第8期790-791,共2页
International Journal of Laboratory Medicine
关键词
树脂类
细菌
厌氧
细菌
需氧
菌血症
微生物敏感性试验
resins
bacteria,anaerobic
bacteria,aerobic
bacteremia
microbial sensitivity tests
