摘要
目的:研究硫芥诱导Hela细胞凋亡的作用,方法:生长在DMEM培养基中的Hela细胞与不同浓度的硫芥作用3小时,凋亡用电镜,电泳及流式术检测.结果:低浓度硫芥(I μmol·L^(-1))抑制细胞生长;较高浓度(1-100 μmol·L^(-1))使细胞主要在G_1期阻滞,发生典型的凋亡形态改变,提取细胞DNA进行琼脂糖凝胶电泳,出现“DNA Ladder”.流式术观察表明硫芥处理3小时后,细胞撤药培养12小时,凋亡率达33％,S期的细胞最敏感,硫芥1000 μmol-L^(-1)使Hela细胞发生明显的坏死,结论:硫芥诱导Hela细胞发生两种不同的损伤:坏死及凋亡,硫芥的细胞毒有浓度依赖性。
AIM: To study the apoptotic effect of sulfur mustard (SM) on Hela cells. METHODS: Exponentially growing Hela cells were treated with SM at various concentrations for 3 h, then apoptosis was examined by electron-microscope, DNA gel electrophoresis, and flow cytometry. RESULTS: SM 1 μmol· L-1 arrested cell growth. After treatment with SM 10 -100 μmol·L-1, cells were mainly blocked at G1-phase with apoptosis. Agarose gel electrophoresis of DNA from cells treated with SM revealed 'DNA Ladder.' About 33 % of the Hela cells showed apoptosis 12 h after 3-h treatment with SM 100μmol· L-1 as determined by flow cytometry and the S-phase cells were more susceptible. However, SM 1000μmol· L-1 caused marked necrosis in Hela cells. CONCLUSION: SM caused 2 distinct forms of cell death, apoptosis or necrosis, in Hela cells in a concentration-dependent manner.
出处
《中国药理学报》
CSCD
1999年第5期445-448,共4页
Acta Pharmacologica Sinica
关键词
细胞凋亡
坏死
芥子气
HELA细胞
硫芥
apoptosis
cell cycle
necrosis
mustard gas
Hela cells
cultured tumor cells
