摘要
目的通过检测食管鳞状细胞癌ｒａｓ、ｐ５３基因的突变状况，探讨基因突变在食管癌发生、发展过程中的意义及对预后的影响。方法采用银染—ＰＣＲ—ＳＳＣＰ法检测４２例食管鳞癌组织中Ｈ—ｒａｓ第１２位密码子及ｐ５３基因５、６、７、８外显子突变的状况。结果２例出现ｒａｓ基因突变。１６例出现ｐ５３基因突变（３８１％）。基因突变与癌的分化程度，淋巴结转移和预后关系密切。结论突变的ｒａｓ、ｐ５３基因可能共同参与了食管癌的发生与进展。突变的ｐ５３基因与食管癌的发展与转移有关并影响预后。
Obective To investigate the effects and the influence on prognosis of gene mutation in production and development of carcinoma of esophagus, by detecting the mutations of ras and p53 genes in 42 patients with esquamous cell carcinoma of esophagus.Methods PCR-SSCP techniques were used to detect the mutations on codon 12 and H-ras genes and exon 5,6,7,8 of p53 genes in squamous carcinoma tissues.Results There were ras gene mutation in 2 cases and p53 gene mutation in 16 cases(38.1%).There were close relationship between the gene mutation and the differentiation of carcinoma,lymphatic metastasis and prognosis.Conclusion Mutations of both ras and p53 genes participate in pioducfion and development of carcinoma of esophagus.There is close interrelation between p53 gene mutation and metastasis of carcinoma of esophgus,which has influence on the prognosis.
出处
《武警医学》
CAS
1999年第4期196-198,共3页
Medical Journal of the Chinese People's Armed Police Force
基金
武警部队1997年立项课题
武警部队科研基金资助
