摘要
建立脑干机械性损伤（肉眼无明显改变）致迅速死亡的动物模型，应用免疫组化、分子生物学、电镜及图像分析等方法，研究脑千损伤的物质基础——神经元、轴索、胶质细胞、血脑屏障。研究结果表明：脑外伤后短时内死亡是脑干损伤的结果，白蛋白、纤维联结蛋白、神经微丝蛋白、胶质纤维酸性蛋白、原癌基因c－Fos等可作为诊断脑干损伤早期病变的指标。在实验研究的基础上，将上述指标应用于怀疑脑干损伤致死案例，验证了这些指标的实用价值，以期为脑外伤后短时间内死亡案例的死因判定提供客观证据，为颅脑损伤的临床救治工作指明重点。
Experimental mechanical brain injury model of rats died within short intervalafter head injury had been established. Changes of neurons, axons, glias cell, and brood-brainbarrier in brain stems were studied by using immunohistochemistry, molecular biology -method, electron microscope and image analysis. The results showed that the cause of deathwas the BSI. A1, Fn, NF, GFAP, and c-fos could .be used as the sensitive diagnostic cri-teria. The established methods for diapnosis of BSI were applied to the forensic medicinepractice.
出处
《中国法医学杂志》
CSCD
1999年第1期9-11,共3页
Chinese Journal of Forensic Medicine
关键词
脑干损伤
病理变化
免疫组化
法医学
Brain-stem injuries Pathological change Immunohistochemisty
