摘要
目的:比较糖尿病与正常家兔的L型钙通道电流(ICa,L),探讨糖尿病家兔在缺血再灌注(I/R)时心室肌细胞ICa,L的变化。方法:糖尿病组以四氧嘧啶制作糖尿病模型,成模后4周行电生理实验;正常组家兔饲养1周后直接行电生理实验;所有家兔均以胶原酶Ⅱ分离单个心室肌细胞。所得细胞分正常对照组和糖尿病对照组(台式液灌流10min)、正常缺血再灌注组和糖尿病缺血再灌注组(缺血液灌流5min,再灌注液灌流5min),每组分别记录基础状态及灌流后10min的ICa,L,比较各组的电流密度。结果:I/R、时间均对ICa,L有影响(P<0.01),糖尿病对ICa,L无明显影响(P>0.05),I/R和时间有交互作用(P<0.01),I/R、时间和糖尿病没有交互作用(P>0.05)。时间-电流密度线上糖尿病缺血再灌注组I/R后直线斜率较正常缺血再灌注组小。结论:I/R后ICa,L减少的程度可能为糖尿病对缺血预适应的影响提供离子通道学证据。
Objective： To compare L-type calcium current in ventricular myocytes between healthy and diabetic rabbits, and discuss the change of L-type calcium current （ICa,L） in diabetic rabbits following ventricular ischemia/reperfusion （I/R）. Methods： The rabbits of normal group were fed for one week before the electrophysiological experiments. The rabbit models of diabetic group were established by tetraoxypyrimidine injection. The electrophysiological experiments were performed four weeks after modeling successfully. The single ventricular myocytes were separated by collagenase Ⅱin both groups of rats. There were 4 groups, normal control group and diabetic control group （perfused 10 minutes with normal extracellular solution）, normal I/R group and diabetic I/R group（perfused 5 minutes with mimic ischemic solution, then perfused 5 minutes with reper-fusion solution）. The currents of L-type calcium were recorded at basic state and 10 min after perfusion. The density currents were compared between different groups. Results： There were significant effects of I/R （P 0.01） and time （P 0.01） on ICa,L, and no significant effect of diabetes on ICa,L （P 0.05）. There was no interaction between IR, time and diabetes, except between I/R and time （P 0.01）. The slope rate on time-density line was smaller in diabetes group than that of normal group. Conclusion： Diabetes didn’t make significant differences in ICa,L, but minify the attenuation degree after I/R, which might provide some clue for the effect of diabetes on I/R.
出处
《天津医药》
CAS
北大核心
2010年第8期700-702,共3页
Tianjin Medical Journal
关键词
糖尿病
实验性
疾病模型
动物
再灌注损伤
肌细胞
心脏
钙通道
L型
兔
diabetes mellitus
experimental disease models
animal reperfusion injury myocytes
cardiac calcium channels
L-type rabbits
