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黄芩醇提取物对牙龈卟啉单胞菌膜泡抑制牙周膜成纤维细胞生物活性的影响 被引量:5

Baicalin extract affects the inhibitory effects of Porphyromonas gingivalis extracellular vesicles on DNA synthesis of human periodontal ligament fibroblasts
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摘要 目的:探讨黄芩醇提取物对牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)膜泡(extracellular vesicles,ECV)抑制牙周膜成纤维细胞(PDLFS)生物活性的影响。方法:采用细胞培养技术、细菌培养技术、3H-TdR掺入法,观察ECV对人牙周膜成纤维细胞DNA合成的抑制作用,以及不同浓度黄芩醇提取物对ECV这一生物活性的阻断作用。结果:细胞培养物中加入50μg/mL ECV时,人牙周膜成纤维细胞DNA的合成受到明显抑制,与空白对照组相比(P<0.05),当同时加入0.5、1、2μg/mL不同浓度黄芩醇提取物时其DNA合成量均有所增加,但以2μg/mL浓度组增加显著,与ECV组相比具有统计学意义(P<0.05)。结论:适宜浓度的黄芩提取物可有效地阻断ECV对HPDLFS生物活性的影响。 AIM:To investigate the effect of baicalin extract on DNA synthesis of human periodontal ligament fibroblasts(HPDLFS) under Porphyromonas gingivalis extracellular vesicles(ECV) stimulation.METHODS:Cultured HPDLFS were stimulated with 50μg/mL ECV or ECV plus different concentrations of baicalin for 36 h.HPDLFS with no stimulation served as the control.3H-TdR incorporation method was used to examine the synthesis of DNA in HPDLFS.RESULTS:DNA synthesis of HPDLFS in ECV group was significantly inhibited(P 0.05).Baicalin dose-dependently increased DNA synthesis of ECV-treated HPDLFS and 2 μg/mL baicalin showd statistically significant stimulating effects(P 0.05).CONCLUSION:Baicalin extract shows inhibitory effect on ECV,suggesting that it might be useful in the treatment of periodontal diseases.
出处 《牙体牙髓牙周病学杂志》 CAS 北大核心 2010年第8期427-429,453,共4页 Chinese Journal of Conservative Dentistry
关键词 黄芩 牙龈卟啉单胞菌 膜泡 牙周膜成纤维细胞 baicalin Porphyromonas gingivalis extracellular vesicles periodontal ligament fibroblasts
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参考文献11

  • 1Mayrand D, Grenier D. Biological activities of outer membrane vesicles [J]. Can JMierobiol, 1989, 35(6) : 607 -613. 被引量:1
  • 2Shah HN, Gharbia SE, Progulske-Fox A, et al. Evidence for independent molecular identity and functional interaction of the haemagglutinin and cysteinc proteinase (gingivain) of Porphyromonas ginglvalis[ J ]. J Med Microbiol , 1992,36(4) :239 - 244. 被引量:1
  • 3倪龙兴,史俊南,陈晓玲,刘智广.成人唾液对牙龈卟啉菌及其提取物毒理学作用的影响[J].中华微生物学和免疫学杂志,1997,17(1):21-21. 被引量:4
  • 4Smalley JW, Birss AJ. Trypsin-ike enzyme activity of the extracellular membrane vesicles of Bacteroides gingivalis W50 [ J]. J Gen Microbiol, 1987, 133 (Pt10) : 2883-2894. 被引量:1
  • 5李德懿.牙周病微生物学新探.国外医学:口腔医学分册,1986,3(15):147-151. 被引量:3
  • 6Holdeman LV, Moore WEC, Cato EP(eds). Anaerobe Laboratory manual [ M]. 4th edn. Blacksburg VA, Virginia polytechinic Institute and state University, 1977 : 1 - 116. 被引量:1
  • 7司徒镇强,吴军正主编..细胞培养[M],1996:363.
  • 8Halhoul N, Colvin JR. The uhrastructure of bacterial plaque attached to the gingiva of man [ J ]. Arch Oral Biol, 1975,20 (2) :115. 被引量:1
  • 9Tsao TF, Newman MG, Kwok YY, et al. Effect of Chinese and western antimicrobial agents on selected oral bacteria [ J ]. J Dent Res ,1982 ,61 (9) :1103 - 1106. 被引量:1
  • 10汤亚玲,谭红,李继遥,肖晓蓉,朱珠,张静仪.黄芩对产黑色素类杆菌内毒素影响的实验研究[J].广东牙病防治,2002,10(2):94-96. 被引量:16

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  • 1Sheets SM, Potempa J, Travis J, et al. Gingipains from Porphyromonas gingivolis W83 induce cell adhesion molecule cleavage and apoptosis in endothelial cells[J]. Infect Immun, 2005, 73 (3) : 1543-1552. 被引量:1
  • 2Loomer PM, Sigusch B, Sukhu B, et al. Direct effects of metabolic products and sonicated extracts of Porphy= romonas gingivolis 2561 on osteogenesis in vitro [J]. Infect Immun, 1994, 62(4):1289-1297. 被引量:1
  • 3Houle MA, Grenier D, Plamondon P, et al. The collagenase activity of Porphyromonos gingivolis is due to Arggingipain[JJ. FEMS Microbiol Lett, 2003, 221 (2): 181- 185. 被引量:1
  • 4Kobayashi-Sakamoto M, Hirose K, Nishikata M, et al. Osteoprotegerin protects endothelial cells against apoptotic cell death induced by Porphyromonas gingivolis cysteine proteinases[JJ. FEMS Microbiol Lett, 2006, 264 (2) : 238- 245. 被引量:1
  • 5Belibasakis GN, Bostanci N, Hashim A, et al. Regulation of RANKL and OPG gene expression in human gingival fibroblasts and periodontal ligament cells by Porphyromonos gingivolis: A putative role of the Arg-gingipains [J]. Microb Pathog, 2007, 43(1):46-53. 被引量:1
  • 6Walters SM, Dubey VS, Jeffrey NR, et al. Antibiotic-induced Porphyromonos gingivolis LPS release and inhibition of LPS-stimulated cytokines by antimicrobial peptides[J]. Peptides, 2010, 31 (9): 1649-1653. 被引量:1
  • 7Bailey MT, Kinsey SG, Padgett DA, et al. Social stress enhances IL-1beta and TNF -alpha production by Porphyromonas gingivolis lipopolysaccharide-stimulated CD Ll h + cells[JJ. Physiol Behav, 2009, 98 (3) : 351- 358. 被引量:1
  • 8Wang PL, Sato K, Oido M, et al. Involvement of CD14 on human gingival fibroblasts in Porphyromonas gingivolis lipopolysaccharide -mediated interleukin -6 secretion [JJ. Arch Oral Bioi, 1998, 43 (9) : 687-694. 被引量:1
  • 9卞金有.口腔预防医学第3版[M].北京:人民卫生出版社,2001.186 - 187. 被引量:5
  • 10寇育荣,潘亚萍.牙龈卟啉单胞菌膜泡诱导牙龈上皮细胞炎性反应的体外研究[J].中华口腔医学杂志,2007,42(9):564-567. 被引量:3

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