摘要
目的:研究血管内皮生长因子(Vascular endothelial growth factor,VEGF)936C/T、-460T/C、405G/C单核苷酸多态性(Single nucleuotide polymorphisms,SNP)与脑梗死(Cerebral infarction,CI)的关系。方法:应用病例对照分析和聚合酶链反应-限制性片段长度多态性方法对150例CI患者与120例健康对照组的VEGF936C/T、-460T/C、405G/C等位基因频率和基因型频率与CI之间的关系进行分析。结果:CI组VEGF936C/T SNP T位点的等位基因频率为0.3667,对照组为0.2500,2组比较有统计学意义(P<0.01);CI组VEGF第936位点的基因频率(CC:34.00%,CT:58.67%,TT:7.33%)与对照组的基因型频率(CC:55.83%,CT:38.33%,TT:5.83%)差异有统计学意义(P=0.001),其中CI组CT+TT基因型频率(66.00%)高于对照组(44.17%,P=0.001);在高血压分层分析后CI组VEGF第936位点的T等位基因频率和CT+TT基因型频率与对照组相比有统计学意义,而两组间-460T/C、405G/C的基因型和等位基因频率的差异无统计学意义。结论:血管内皮生长因子VEGF936C/T位点与CI有关联,可能是CI的独立危险因素,-460T/C、405G/C与CI无关联。
Objective：To study the relationship between the single nucleotide polymorphisms （SNP）936C/T,-460T/C,405G/C in Vascular endothelial growth factor （VEGF）gene and their association with cerebral infarction （CI）.Methods：Case-control study was carried out to investigate allelic genotypes and frequency of the three VEGF SNP in 150 subjects with CI and 120 control individuals.Polymerase chain reaction-Restriction fragment lengthpolymorphism,（PCR-RFLP）[U3] and DNA sequencing were used to analyze the polymorphisms.The correlations between VEGF SNPs and CI were evaluated.Results：There was a highly significant difference in allele frequency of 936C/T SNP between CI and control subjects,and the T allele frequencies were respectively 0.3667 versus 0.2500（P0.01）.The frequencies of 936C/T SNP genotypes were significantly different（P=0.001）between the CI group（CC：34.00%,CT：58.67%,TT：7.33%）and the control group （CC：55.83%,CT：38.33%,TT：5.83%）.The frequency （66.00%）of genotype （CT＋TT）of 936C/T SNP in CI group were significantly higher than that （44.17%）in the control group （P=0.001）.The frequencies of allele T and genotype （CT＋TT）of VEGF936C/T SNP were still associated with CI after stratification of blood pressure.No difference was found in the genotype and allelic frequencies of VEGF-460T/C,405G/C alleles between CI group and the control groups.Conclusion：Our results demonstrated that VEGF936C/T SNP might be associated with CI and be an independent risk factor of CI.But we did not find that VEGF-460T/C,405G/C SNP associated with CI.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2010年第7期1014-1017,共4页
Journal of Chongqing Medical University
关键词
血管内皮生长因子
单核苷酸多态性
脑梗死
Vascular endothelial growth factor
Single nucleotide polymorphisms
Cerebral infarction
