摘要
目的：研究肾小管和内皮素与肾间质纤维化的关系。方法：应用肾小管上皮细胞及肾成纤维细胞的体外培养，通过逆转录多聚酶链反应，免疫组织化学染色，放射免疫测定及双重免疫组织化学染色技术，并测定３ＨＴｄＲ掺入率，观察肾小管损伤时产生的内皮素１（ｅｎｄｏｔｈｅｌｉｎ１ＥＴ１）与肾成纤维细胞增殖的关系。结果：肾小管上皮细胞既有ＥＴ１ｍＲＮＡ的表达，又有ＥＴ的两种受体（ＥＴＡＲｍＲＮＡ和ＥＴＢＲｍＲＮＡ）的表达，还有ＥＴ１的蛋白合成及分泌。且肾小管损伤及再生过程中，ＥＴ１表达增加。对体外培养的大鼠肾成纤维细胞加入ＥＴ１，３ＨＴｄＲ掺入率较对照组明显增高。并证明肾成纤维细胞有ＥＴＲｍＲＮＡ的表达。结论：损伤及再生的肾小管上皮细胞较正常时合成和分泌更多的ＥＴ１，ＥＴ１通过ＥＴＲ的作用，促使肾成纤维细胞的增殖。
Objective: To study the relationship between injured renal tubular epithelial cell, endothelin(ET), and renal tubulointerstitial fibrosis(TIF). Methods: Renal tubular epithelial cell and renal fibroblast were cultured in vitro , and reverse transcription polymerase chain reaction(RT PCR), immunohistochemical stain(IMC), radioimmuno assay (RIA) and double IMC were used, and the ratio of 3H TdR incorporation was measured to observe the relationship between ET 1 and proliferation of renal fibroblast. Results: There existed the expression of ET 1 mRNA and its receptors (ET A R mRNA and ET B R mRNA), and also the synthesis of ET 1 peptide in renal tubular epithelial cell. In the course of damage and regeneration, renal tubular epithelial cell produced more ET 1. The ratio of 3H TdR incorporation of renal fibroblast was higher than that of control group when ET 1 to was added this cultured cell. There existed the expression of ET B R mRNA in renal fibroblast. Conclusion: Injured and regenerated renal tubular epithelial cell produced and secreted more ET 1 than in normal kidney. ET 1 contributed to interstitial fibrosis by promoting the proliferation of renal fibroblast through ET R.
出处
《北京医科大学学报》
CSCD
1999年第1期23-26,共4页
Journal of Peking University(Health Sciences)
基金
国家自然科学基金
