摘要
目的：研究白细胞介素-2基因转染人骨肉瘤细胞体外生物学特性．方法：应用逆转录病毒载体pDOR－neo将人的IL－2cDNA插入人骨肉瘤细胞，经G418筛选及PCR分析获得阳性细胞，并测定其上清液中IL－2含量．结果：PCR可从阳性细胞基因组中扩增出IL－2基因片段，并从其上清液中测得每24h105细胞IL－2活性为50U～800U．结论：逆转录病毒介导的IL－2CDNA可以在人骨肉瘤细胞系中获得有效表达，且转基因后的细胞较原野生型细胞体外生物学特性有明显差别．
AIM: To study in vitro biological characteristic ofhuman osteosarcoma cells transfected with IL-2 cDNA.METHODS: The human IL- 2 cDNA was inserted into the human osteosarcoma cells with the retroviral vector pDOR-neo.The human osteosarcoma cells secreting IL-2 were abtainedafter G418 screening and analysed by PCR. RESULTS: PCRwas used to identify the integration of the IL-2 gene in genomic DNA of G418-resistant human osteosarcoma cells. Theproduction of IL-2 was 50 U--800 U per 1×105 cells every 24h. CONCLUSION: IL-2 cDNA could be efficiently expressivein the tranduced human osteosarcoma cells and the resultsdemonstrated the in vitro biological characteristics of osteosarcoma cells transfected IL-2 gene were obviously different from the wild type ostosarcoma cells.
出处
《第四军医大学学报》
1999年第2期134-136,共3页
Journal of the Fourth Military Medical University
