摘要
目的：检测口腔鳞癌组织中３ｐ１４．２区Ｄ３Ｓ１３００位点丢失情况，为新的抑癌基因定位提供依据。方法：应用聚合酶链反应（ＰＣＲ）技术以Ｄ３Ｓ１３００位点微卫星多态为遗传标记，检测口腔鳞癌组织中该位点的杂合性丢失（Ｌｏｓｏｆｈｅｔｅｒｏｚｙｇｏｓｉｔｙ，ＬＯＨ）状况。结果：２２例口腔鳞癌１７例提供信息，７例出现杂合性丢失，ＬＯＨ率为４１％（７／１７），有２例出现微卫星的不稳定性。结论：３ｐ１４．２区Ｄ３Ｓ１３００位点杂合性丢失可能与口腔鳞癌的发生发展相关，提示该区域可能存在口腔鳞癌的抑癌基因。
Objective: To discuss the potential loss of tumor suppressor gene loci on chromosome 3p14.2 in oral squamous cell carcinoma (OSCC) and to provide meterials for locating new suppressor gene. Methods: We analyzed 22 pairing microdissected samples of normal tissues and OSCC from the same patients. LOH was detected by examing microsatellite polymorphic sequence in loci D3S1300 (3p14.2) by using PCR. Results: In 7 of 17 (41%), LOH was found at 3p14.2 (D3S1300). Microsatellite instability was found in two OSCC. Conclusion: This suggested that there was frequent loss on 3p14.2 in OSCC. There probably exist a suppressor gene which is important in oncogenesis and progression of OSCC. The mismatch repair gene which belongs to neither oncogene nor suppressor gene probably also plays an important role in OSCC.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1999年第1期36-37,共2页
Journal of China Medical University
关键词
鳞状细胞癌
口腔癌
抑癌基因
杂合性丢失
染色体
squamous cell carcinoma
oral cancer
tumor suppressor gene
loss of heterozygosity
