摘要
采用砂培盆栽试验探讨施Se对药用菊花花中总黄酮、绿原酸和Se含量的影响。结果表明,在施Se量不超过2.0mg/kg时能促进药菊的生长,提高药菊花中总黄酮、绿原酸的含量,药菊花、茎叶和根的干重和花中的总黄酮、绿原酸含量均随施Se量的增加而增加;其中以施Se量为2.0mg/kg处理的效果最佳,该施Se量处理药菊花干重、根干重、花中绿原酸含量与施Se量分别为0、0.25、0.5、1.0mg/kg各处理以上各相应指标的差异均达到显著水平,该施Se量处理药菊花中总黄酮含量高于其他所有5处理,并与施Se量为0mg/kg和0.25mg/kg两处理药菊花中总黄酮含量的差异达到显著水平。过量施Se(4.0mg/kg)对药菊的生长表现出一定抑制作用,其药菊各部位生物量和花中总黄酮、绿原酸含量均低于施Se量2.0mg/kg的处理。药菊花、茎叶和根中的Se含量均随施Se量的增加而增加,菊花茎叶和根中的Se含量与施Se量呈线性相关关系,而菊花花中的Se含量(y)与施Se量(x)符合一元二次回归方程:y=-0.8175x2+6.0045x+0.1363。施用适量的Se既能显著提高药菊花中总黄酮和绿原酸含量,又能大幅提高花中的Se含量,这为富Se菊花及其系列产品的研发提供了理论依据。
In order to investigate the effects of different application does of selenium （Se） on the contents of total flavonoid, chlorogenic acid and selenium in the flower of Chrysanthemum morifolium, a sand culture experiment was conducted. The results showed that, it promoted the plant growth and increased the contents of total flavonoid, chlorogenic acid, when Se applying doses did not exceed 2.0 mg/kg. The dry weights of flower, stem-leaf, root and the contents of total flavonoid, chlorogenic acid increased with the increase of Se applying doses, the optimal effect was obtained when Se at 2.0 mg/kg. The dry weight of flower, root and the contents of chlorogenic acid of the plants in the treatment of Se at 2.0 mg/kg were significantly higher than Se applications at 0, 0.25, 0.5 mg/kg, and the former＇ contents of total flavonoid was higher than Se application at 0, 0.25, 0.5, 1.0, 4.0 mg/kg. Excessive Se applying dose, such as at 4.0 mg/kg inhibited the growth of the plants, the dry weight of flower, stcm-leaf, root and the contents of total flavonoid, chlorogenic acid were lower than Se at 2.0 mg/kg. The contents of Se in flower, stem-leaf and root of Chrysanthemum morifolium increased with the increase of Se application doses. The relationship between Se contents in stem-leaf and root of Chrysanthemum morifolium and Se application rate is liner, while the relationship between Se content in flower（y） and Se application rate（x） fits an equation （y = -0.817 5x2 ＋ 6.004 5x ＋ 0.136 3）. It showed that the suitable Se applying doses could significantly increase the contents of total flavonoid, chlorogenic acid and Se content in the flower. The above results provide the theoretical basis for the development of Se-enriched Chrysanthemum morifolium and its products.
出处
《土壤》
CAS
CSCD
北大核心
2010年第4期618-623,共6页
Soils
基金
国家科技部农业科技成果转化资金项目(2006GB2C100097)
江苏省科技攻关项目(BE2006338)资助
关键词
药用菊花
施硒
总黄酮
绿原酸
富硒菊花
Chrysanthemum morifolium, Selenium application, Total flavonoid, Chlorogenic acid, Selenium-enriched Chrysanthemum morifolium
