摘要
目的研究乙型肝炎病毒血清前S1抗原、HBV-DNA与其他乙型肝炎血清标志物HBs-Ag、HBeAg的相关性。方法采用实时聚合酶链反应(Real-Time PCR)技术检测血清中HBV-DNA的含量,用酶联免疫法(ELISA)检测PreS1Ag、HBsAg和HBeAg。采用SPSS 13.0统计软件进行分析,成组设计资料,率比较采用配对的χ2检验,结果的关联性采用独立性的χ2检验。结果以HBV-DNA<1.000E+03Copy/ml为阴性组;HBV-DNA≥1.000E+03Copy/ml为阳性组。600例HBsAg阳性的乙型肝炎患者中241例HBV-DNA阳性,283例Pre-S1阳性。其中241例HBV-DNA阳性患者中,Pre-S1阳性222例,阳性率为92.12%。显著高于HBV-DNA阴性组的Pre-S1阳性率(16.99%)。经配对的差异性检验P=0.000(确切概率)。经独立性检验,χ2=326.573,P=0.000。241例HBsAg、HBV-DNA阳性组,HBeAg阳性191例,Pre-S1阳性222例,其中191例HBeAg阳性患者中,Pre-S1Ag阳性185例,阳性率为96.86%。显著高于HBeAg阴性组的Pre-S1Ag阳性率(61.67%)。经配对的差异性检验P=0.000(确切概率)。经独立性检验,χ2=28.511,P=0.000。结论 Pre-S1Ag与HBV-DNA阳性高度相关,有好的一致性和互补性,较HBeAg更敏感。可作为乙肝病毒存在和复制的可靠标志,是反映HBV是否具有传染性的观察指标。
Objective To investigate the correlation between Hepatitis B virus Pre-S1 antigen,HBV-DNA and Hepatitis B s antigen（HBsAg）,Hepatitis B e antigen（HBeAg） in serum.Method HBV-DNA was determined by Real Time Polymerase Chain Reaction（Real-Time PCR）.Enzyme-Linked Immunosorbent Assay（ELISA） method was used to detect Pre-S1Ag,HBsAg and HBeAg in serum.SPSS 13.0 statistics Sofeware,group design and χ2 test were used for result analysis.Result In the 600 HBs antigen positive patients,241 were HBV-DNA positive（HBV-DNA ≥ 1.000E＋03 copy/ml,as the positive group）,283 were Pre-S1 antigen positive.In the 241 HBV-DNA positive cases,222 were Pre-S1 antigen positive（92.12%）,significantly higher than that in the HBV-DNA negative（HBV-DNA 1.000E＋03 copy/ml） group（16.99%）.The difference between paired test P=0.000（exact probability）.The independence test χ2=326.573,P=0.000.In the 241 cases of Hepatitis B s antigen positive in HBV-DNA positive group,191 were hepatitis B e antigen positive,185 of which were Pre-S1 antigen positive（96.86%）,significantly higher than that in the hepatitis B e antigen negative group（61.67%）.The difference between paired test P=0.000（exact probability）.The independence test χ2=28.511,P=0.000.Conclusion The positive rate of Pre-S1 antigen is highly correlated with the positive rate of HBV-DNA with better consistency and complementarity,more sensitive than the HBe antigen.It can be used as a reliable index of existence and replication of hepatitis B Virus,and as an indicator of HBV infectivity.
出处
《中国微生态学杂志》
CAS
CSCD
2010年第8期719-721,共3页
Chinese Journal of Microecology
