摘要
目的检测酪氨酸激酶抑制剂PTK787对急性髓系白血病小鼠血清VEGF浓度及其受体VEGFR-2 mRNA表达水平的影响,进一步探讨PTK787抗急性髓性白血病的作用机制。方法建立急性髓系白血病(AML)SCID小鼠模型,采用酶联免疫吸附试验(ELISA)和反转录PCR(RT-PCR)分别动态检测不同时间点正常小鼠、白血病小鼠及PTK787治疗组小鼠外周血清VEGF浓度及其受体VEGFR-2 mRNA表达的水平变化。结果 (1)正常小鼠和患病小鼠均表达VEGF及VEGFR-2 mRNA;(2)患病组外周血清VEGF浓度和VEGFR-2 mRNA的表达均显著高于正常组小鼠(P<0.05),而且随着病程的进展逐渐升高;(3)PTK787治疗组小鼠外周血清VEGF浓度及其受体VEGFR-2 mRNA表达的水平均较患病组明显降低(P<0.05)。结论 PTK78抗急性髓性白血病的作用与降低血清VEGF及其受体VEGFR-2 mRNA表达的水平密切相关。
Objective To determine the effect of PTK787 on the expression of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 mRNA,and further discuss the role of PTK787 on anti-acute myeloid leukemia.Methods The acute myeloid leukemia model was established on 40 severe combined immunodeficiency mice by HL-60 cells transplantation.The mice were divided into five group randomly,the normal group,the sicken group,the treated group with 50mg/kg PTK787,the treated group with 100mg/kg PTK787,the treated group with 200mg/kg PTK787.Logarithmic phase cells were implanted into the sicken group and the treated group by celiac injection.The expression of vascular endothelial growth factor was detected by enzyme linked immunosorbent assay.The expression of vascular endothelial growth factor receptor-2 mRNA was detected by reverse transcription-polymerase chain reaction.Results（1）Expression of vascular endothelial growth factors and vascular endothelial growth factor receptor-2 mRNA were determined on all mice.（2）Compared with the normal group,the mRNA level of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 in the sicken group was significantly and gradually increased with the course of disease.（3）Compared with the sicken group,the expression of vascular endothelial growth factor and vascular endothelial growth factor receptor-2 mRNA of treated group decreased obviously.Conclusion The anti-effect on acute myeloid leukemia of PTK787 is related with the decrease expression of vascular endothelial growth factor and vascular endothelial growth factor receptor-2.
出处
《肿瘤防治研究》
CAS
CSCD
北大核心
2010年第8期859-863,共5页
Cancer Research on Prevention and Treatment
基金
广东省卫生厅资助项目(A2003556)
