摘要
目的研究肺癌组织中脆性组氨酸三位体（ＦＨＩＴ）基因的改变。方法采用逆转录聚合酶链反应（ＲＴＰＣＲ）和逆转录聚合酶链反应单链构象多态性（ＲＴＰＣＲＳＳＣＰ）方法分析４７例肺癌组织和其中１６例相应的转移性肺门淋巴结。结果６８％（３２／４７）肺癌原发灶（包括２例肺鳞状细胞原位癌）和９４％（１５／１６）转移性肺门淋巴结出现ＦＨＩＴ转录本缺失，二者统计学差异有显著性（Ｐ＜０．０５）。ＦＨＩＴ基因转录本缺失主要发生于编码区。ＲＴＰＣＲＳＳＣＰ分析未发现突变。结论（１）在肺癌组织中ＦＨＩＴ基因转录本缺失是频发事件，且可能为早期事件；（２）ＦＨＩＴ基因突变可能是少见事件；（３）转移性肺门淋巴结ＦＨＩＴ基因转录本缺失频率与肺癌原发灶相比差异有显著性。
Objective To investigate the significance of the fragile histidine triad (FHIT) gene in evaluation of early diagnosis and metastasis in lung cancer. Methods 47 lung cancer samples and 16 matched metastatic hilar lymphnodes of lung cancer patients were examined for abnormalities of the gene by using RT PCR and RT PCR SSCP. Results 32 of 47(68%)lung cancer samples and 15 of 16 metastatic hilar lymphnodes showed loss of FHIT gene transcripts (exon5 9 or exon1 4). RT PCR SSCP reveal no variants. Conclusions These data indicate that loss of FHIT gene transcript is frequent in lung cancer and may be an early event in lung tumorigenesis and FRA3B may be involved in the development and progression of the lung cancer.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1999年第3期159-162,共4页
Chinese Journal of Tuberculosis and Respiratory Diseases
基金
国家攀登计划项目资金
