摘要
目的：研究促皮质素（Ｃｏｒ）对甲醛引起大鼠脊髓内生长抑素及其合成的影响．方法：采用免疫组织化学，免疫组织化学双重染色法和原位杂交技术．结果：足底注射甲醛使大鼠脊髓背角内ＦＬＩ，ＳｏｍＬＩ，ＳｏｍＬＩ／ＦＬＩ和ＰＰＳｍＲＮＡ神经元数均较对照组显著增多．单独ｉｐＣｏｒ对脊髓背角ＦＬＩ和ＳｏｍＬＩ无明显影响．但是，ｉｐＣｏｒ显著抑制甲醛引起的背角内ＦＬＩ，ＳｏｍＬＩ，ＳｏｍＬＩ／ＦＬＩ和ＰＰＳｍＲＮＡ神经元数的增多效应，并有量效关系．中脑中缝核内预先注射赛庚啶阻断这一抑制效应，而荷包牡丹碱，纳洛酮，酚妥拉明则无影响．结论：ｉｐＣｏｒ通过５ＨＴ受体抑制甲醛引起的大鼠脊髓背角内ｃｆｏｓ表达，Ｓｏｍ和Ｓｏｍ合成而发挥镇痛作用．
AIM: To study the effect of corticotrophin (Cor) on somatostatin (Som) and its synthesis in spinal dorsal horn induced by formaldehyde in rats. METHODS: Using double immunohistochemical stainings, and in situ hybridization. RE￣SULTS: Two hours after sc formaldehyde ( 5 % , 200 μL) in one hindpaw of rats, the neurons of c fos like immunoreactivity (FLI), somatostatin like immunoreactivity (Som LI), Som LI/FLI, and perprosomatostatin mRNA (PPS mRNA) in ipsilateral spinal dorsal horn were increased obviously, as compared with the control group. The FLI and Som LI of spinal cord were not changed by ip Cor. Cor (25 or 12 5 U·kg -1 , ip) inhibited the formaldehyde evoked FLI, Som LI, Som LI/FLI, and PPS mRNA of spinal cord in a dose dependent manner. The decrease of c fos or Som level due to ip Cor in rats of chronic pain was prevented by raphe nuclei injected cyproheptadine, but not by bicuculline, naloxone, or phentolamin injected to raphe nuclei. CONCLUSION: The formal￣dehyde evoked c fos expression, Som, and Som synthesis of spinal cord were suppressed by Cor through the serotonin receptor of raphe nuclei.
出处
《中国药理学报》
CSCD
1999年第2期188-192,共5页
Acta Pharmacologica Sinica
关键词
促皮质素
生长抑素
甲醛
脊髓
免疫组织化学
corticotropin
cyproheptadine
naloxone
phentolamine
analgesia
spinal cord
proto oncogene proteins c fos
somatostatin
immunohistochemistry
in situ hybridization
