摘要
目的：研究榄香烯的抗肿瘤作用机制．方法：抑制细胞增殖采用ＭＴＴ法；用荧光显微镜观察细胞的形态学变化；ＤＮＡ电泳、流式细胞仪技术检测ＤＮＡ断裂；用流式细胞仪检测ｂｃｌ２蛋白的表达．结果：榄香烯６５－５２０μｍｏｌ·Ｌ－１明显抑制Ｋ５６２细胞增殖，ＩＣ５０（９５％可信区间）为２２０（１５２－３１９）μｍｏｌ·Ｌ－１，电泳可见ＤＮＡ断裂形成的阶梯状条带，形态学上表现为染色体聚集，核固缩、断裂，而ｂｃｌ２蛋白的水平明显下降．结论：榄香烯诱导人白血病Ｋ５６２细胞凋亡。
AIM: To study the antitumor action of elemene (Ele) and its mechanism. METHODS: Inhibition of proliferation was measured with a colorimetric 3 [4,5 dimethyl thiazol 2 yl] 2,5 diphenyltetra￣zolium bromide (MTT) assay. Mor￣phological assessment of apoptosis was performed with fluorescence microscope. DNA frag￣menta￣tion was assessed by agarose gel electrophoresis and flow cytometry. The levels of bcl 2 protein was measured with flow cytometry. RESULTS: Exposure of exponen￣tial￣ly growing K562 cells to Ele 65-520 μmol·L -1 for 48 h resulted in growth arrest. The values of IC 50 and 95 % confidence limits were 220 (152-319) μmol·L -1 . After treatment of K562 cells with Ele 130 μmol·L -1 , marked morphological changes including “Apo bodies” reduction in volume were observed with fluorescence microscope. Agarose gel electro￣phoresis of DNA from cells treated with Ele for 48 h revealed “ladder” pattern. The levels of bcl 2 protein in K562 cells treated with Ele for 48 h were obviously decreased. CONCLU￣SION: Ele induces apoptosis of K562 cells, which is related with the down regulation of bcl 2 protein in K562 cells.
出处
《中国药理学报》
CSCD
1999年第2期103-106,共4页
Acta Pharmacologica Sinica
关键词
榄香烯
白血病
K562
细胞凋亡
BCL-2
基因
elemene
leukemia K562
apoptosis
bcl
2 genes
DNA damage
cultured tumor cells
agar gel electrophoresis
flow cytometry
