摘要
将基于液相色谱-质谱联用(LC-MS)技术的代谢组学分析平台用于薄荷烟对大鼠代谢影响的研究。分析了3组大鼠的尿样,包括对照大鼠、吸食普通烟大鼠和吸食薄荷烟大鼠,并采用主成分分析(PCA)方法对数据进行模式识别。PCA得分图表明吸食薄荷烟大鼠与对照组大鼠尿样的代谢差异要小于吸食普通烟大鼠。从PCA载荷图中找到并鉴定了犬尿喹啉酸等8种重要代谢物。通过考察代谢物在对照大鼠、吸食薄荷烟大鼠和吸食普通烟大鼠尿样中的相对含量变化,进一步说明了烟草中添加薄荷醇可减少烟草对大鼠代谢的影响。
A liquid chromatography-mass spectrometry （ LC-MS） method was developed for the metabo-nomics study of menthol cigarette effect on rats. Urines from three groups of rats were analyzed,including control rats,rats treated with menthol cigarette and rats treated with normal cigarette,and the data were processed by the method of principal component analysis （ PCA） . The PCA score plot showed that the metabolic difference between the rats treated with menthol cigarette and the control rats was smaller than that between the rats treated with normal cigarette and the control rats. Based on the PCA score plot, eight important metabolites,for example,kynurenic acid,were found and identified. Their relative con-centration changes among the three groups further indicated that menthol cigarette maybe decrease the metabolic effect on rats.
出处
《色谱》
CAS
CSCD
北大核心
2010年第8期765-768,共4页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(No.20805045)
湖南中烟工业公司科技项目
关键词
液相色谱-质谱联用
代谢组学
薄荷烟
大鼠
liquid chromatography-mass spectrometry （ LC-MS）
metabonomics
menthol cigarette
rats
