摘要
在冷冻条件下,利用7230分光光度计、显微摄影及IEF-PAGE等方法测定不同浓度的DMSO对桃树叶肉细胞的抗冻作用。发现(1)材料冷冻后再加入DMSO对细胞的抗冻作用不明显;(2)先用DMSO处理后对细胞有明显的抗冻作用,其中VDMSO∶VH2O=2∶3及VDMSO∶VH2O=1∶1的配比对桃树叶肉细胞有较好的效果。
This experiment utilize such guotes as 7230-Visible spectophometer microphotogrophy.IEF-PAGE etc.,and dralis support from chart,data and photoes. It probes into the frost-poof action of DMSO to the mesophyll cells and we found:(1) The DMSO has no evident action to the cell after been frosted.(2) after handled by the DMSO. The plant cell has clear frost-proof action especially the F group which is the best in all the fest group. (3) The DMSO clots harm for the cell if the concertration as very high such as G group.
出处
《安徽大学学报(自然科学版)》
CAS
1999年第2期99-102,共4页
Journal of Anhui University(Natural Science Edition)
关键词
二甲亚砜
桃树
叶肉细胞
抗冻性
冻害
dimefhyl,sulfoxic,mesophyll cess,frost-proof characteristics