摘要
模拟豆科植物根瘤在固氮过程中释放氢气,分析其对根际土壤产生的影响.从黄土高原人工刺槐林采集有根瘤的刺槐根际土壤样品,采用电解水产生氢气分次处理,对处理后与未处理的土壤样品内微生物种群数量、微生物代谢强度及土壤脱氢酶、过氧化氢酶、脲酶、转化酶等指标进行比较分析.氢气处理后的两批土样中,氢氧化细菌数量均显著增多,分别占9月、5月土壤微生物总量的34.9%和32.4%;微生物在土壤中代谢强度分别增加了140.2%和57.6%;土壤脱氢酶、过氧化氢酶、脲酶和转化酶含量均表现增加,其中9月处理土壤分别增加36.6%、57.1%、26.6%和147.0%,5月处理土壤分别增加37.2%、6.6%、108.8%和147.0%.研究结果为根瘤固氮过程中产生氢气对根际土壤微环境的影响及促生机制提供了实验资料.
In the nitrogen-fixation process,hydrogen is released and affects plant rhizosphere soil.Soil samples containing locust nodules were collected from artif icial Robinia pseudoacacia forest of the Loess Plateau in China in September 2008 and May 2009,and treated by hydrogen produced with electrolysis of water 30 days later.The number of soil microbial populations,microbial metabolisability,and soil dehydrogenase,catalase,urease and invertase contents were determined and analyzed.The results showed that the number of hydrogen-oxidizing bacteria increased distinctly in the treated soil,accounting for 34.9% in September and 32.4% in May of the total soil microorganisms.Microbial metabolism intensity in the treated soil was increased by 140.2% and 57.6% than that in the untreated soil.Dehydrogenase,catalase,urease and invertase contents in the treated soil were also increased,by 36.6%,57.1%,26.6% and 147.0% than those in the untreated soil in September 2008 and by 37.2%,6.6%,108.8%,147.0% in May 2009.The results indicated the hydrogen produced during nitrogen-fixation would affect the rhizosphere soil micro-environment and the growth-promoting mechanism.Fig 2,Tab 3,Ref 14
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2010年第4期515-518,共4页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学重点基金项目(No.30630054)资助~~
关键词
氢气
根际土壤
氢氧化细菌
微生物代谢强度
土壤酶
根瘤固氮
刺槐
hydrogen
rhizosphere soil
hydrogen-oxidizing bacteria
microbial metabolism intensity
soil enzyme
nitrogenfixation
Robinia pseudoacacia
