摘要
以外消旋扁桃酸为唯一碳源,从土壤中筛选得到一株选择性降解S-扁桃酸的铜绿假单胞菌,可用于选择性降解S-扁桃酸制备R-扁桃酸。考察了温度、pH值、底物浓度等因素对S-扁桃酸降解的影响,并对发酵培养基成分及转化条件进行了优化。结果表明,在35℃、pH值8.0、底物浓度131.4 mmol.L-1的优化条件下,68 h发酵完成,R-扁桃酸收率为48.2%、光学纯度为99.2%。
A mandelate-degrading bacterium,Pseudomonas aeruginosa,was isolated from soil using racemic mandelic acid as sole carbon source.It could degrade S-mandelic acid with high enantioselectivity and obtain R-mandelic acid.This study examined the factors which affecting biotransformation efficiency,including temperature,pH value and substrate concentration,and optimized the component of the culture medium and the transformation conditions.Shaking cultured for 68 h under the optimum conditions as follows：substrate concentration 131.4 mmol·L^-1,temperature 35℃,pH value 8.0,the fermentation came to the end and 48.2% of yield and 99.2% of optical purity for R-mandelic acid were obtained.
出处
《化学与生物工程》
CAS
2010年第8期66-69,共4页
Chemistry & Bioengineering
关键词
铜绿假单胞菌
R-扁桃酸
对映选择性降解
Pseudomonas aeruginosa
R-mandelic acid
enantioselective degradation
