摘要
A rapid, ultrasensitive and convenient fluorescence measurement technology based on the enhancement of the fluorescence intensity resulting from the interaction of functionalized CdSe/CdS quantum dots （QDs） with bov/ne serum albumin （BSA） was proposed. The citrate-stabilized CdSe/CdS （QDs） were synthesized by using Se powder and Na2S as precursors instead of any pyrophoric organometallic precursors. The modified CdSe/CdS QDs are brighter and more stable against photobleaching in comparison with organic fluorophores. At pH 7.0, the fluorescence signal of CdSe/CdS is enhanced by increasing the concentration of BSA in the range of 0.1-10 μg/mL, and the low detection limit is 0.06 μg/mL. A linear relationship between the enhanced fluorescence peak intensity （△F） and BSA concentration （c） is established using equation △F=50.7c＋16.4 （R=0.996 36）. Results of determination for BSA in three synthetic samples are identical with the true values, and the recovery （98.9%-102.4%） and relative standard deviation （RSD, 1.8%-2.5%） are satisfactory.
A rapid,ultrasensitive and convenient fluorescence measurement technology based on the enhancement of the fluorescence intensity resulting from the interaction of functionalized CdSe/CdS quantum dots(QDs) with bovine serum albumin(BSA) was proposed.The citrate-stabilized CdSe/CdS(QDs) were synthesized by using Se powder and Na2S as precursors instead of any pyrophoric organometallic precursors.The modified CdSe/CdS QDs are brighter and more stable against photobleaching in comparison with organic fluorophores.At pH 7.0,the fluorescence signal of CdSe/CdS is enhanced by increasing the concentration of BSA in the range of 0.1-10 μg/mL,and the low detection limit is 0.06 μg/mL.A linear relationship between the enhanced fluorescence peak intensity(-F) and BSA concentration(c) is established using equation-F=50.7c+16.4(R=0.996 36).Results of determination for BSA in three synthetic samples are identical with the true values,and the recovery(98.9%-102.4%) and relative standard deviation(RSD,1.8%-2.5%) are satisfactory.
基金
Project(50772133) supported by the National Natural Science Foundation of China
