摘要
目的通过对常染色体和X染色体STR基因座以及线粒体DNA高变区多态性的检验,探讨同母异父半同胞关系鉴定策略。方法提取3名全同胞及其1名疑似同母异父半同胞个体的DNA,采用SinofilerTM试剂盒检测常染色体上的15个STR基因座、采用Mentype誖Argus X-8试剂盒和自主研制的16重X染色体STR扩增体系,共检验X染色体上的19个STR基因座,同时采用基因测序技术分析线粒体DNA高变区Ⅰ和高变区Ⅱ的多态性。结果依据常染色体STR基因型结果及全同胞指数和半同胞指数计算结果排除可疑个体与已知3名全同胞间存在全同胞关系,线粒体DNA高变区多态性检测结果提示4名被鉴定人为同一母系,X染色体STR分型结果支持被鉴定个体间为半同胞关系。结论对于同母异父半同胞鉴定案例,综合运用常染色体STR、X染色体STR及线粒体DNA高变区测序等多种遗传分析手段,可获得可靠的鉴定结论。
Objective Determination strategies for half sibling sharing a same mother were investigated through the detection of autosomal and X-chromosomal STR（X-STR） loci and polymorphisms on hypervariable（HV） region of mitochondrial DNA（mtDNA）.Methods Genomic DNA were extracted from blood stain samples of the 3 full siblings and one dubious half sibling sharing the same mother with them.Fifteen autosomal STR loci were genotyped by SinofilerTM kit,and 19 X-STR loci were genotyped by Mentype Argus X-8 kit and 16 plex in-house system.Polymorphisms of mtDNA HV-Ⅰ and HV-Ⅱ were also detected with sequencing technology.Results Full sibling relationship between the dubious half sibling and each of the 3 full siblings were excluded based on the results of autosomal STR genotyping and calculation of full sibling index（FSI） and half sibling index（HIS）.Results of sequencing for mtDNA HV-Ⅰ and HV-Ⅱ showed that all of the 4 samples came from a same maternal line.X-STR genotyping results determined that the dubious half sibling shared a same mother with the 3 full siblings.Conclusion It is reliable to combine three different genotyping technologies including autosomal STR,X-STR and sequencing of mtD-NA HV-Ⅰ and HV-Ⅱ for determination of half sibling sharing a same mother.
出处
《法医学杂志》
CAS
CSCD
2010年第4期279-281,300,共4页
Journal of Forensic Medicine
基金
中央级科研院所社会公益研究资助项目(GY0902)
