摘要
目的分析血浆肿瘤坏死因子受体相关因子-3(tumor necrosis factor receptor-associated fac-tor-3,TRAF3)水平对于炎症性肠病的诊断价值以及与内镜下疾病活动性的相关性。方法应用酶联免疫吸附试验(Enzyme-Linked ImmunoSorbent Assay,ELISA)分析炎症性肠病患者和正常对照者血浆中TRAF3蛋白的表达,受试者工作特征曲线(receiver-operating characteristic,ROC)分析血浆TRAF3水平对于克罗恩病和溃疡性结肠炎的诊断价值,应用Pearson相关分析研究血浆TRAF3水平与内镜下疾病活动性的相关性。数据处理使用GraphPad Prism 5。结果克罗恩病患者(P=0.000)和溃疡性结肠炎患者(P=0.000)血浆TRAF3水平显著高于正常对照者,同时TRAF3对区分克罗恩病患者和正常对照者(P=0.000)以及区分溃疡性结肠炎患者和正常对照者(P=0.000)具有显著的诊断价值,克罗恩病患者(r=-0.093,P=0.473)以及溃疡性结肠炎患者(r=0.043,P=0.733)血浆TRAF3表达水平和内镜下疾病活动指数均无显著的相关性。结论炎症性肠病患者血浆TRAF3水平增高,对区分炎症性肠病患者和正常对照者具有诊断价值,但血浆中TRAF3的水平不能反应内镜下疾病活动程度。
Objective To analyze the diagnostic value of plasma tumor necrosis factor receptor-associated factor-3（TRAF3）in inflammatory bowel disease and its correlation with endoscopic disease activity.Methods Enzyme-Linked ImmunoSorbent Assay（ELISA）was applied to evaluate plasma TRAF3 levels in patients with inflammatory bowel disease and in healthy controls.Receiver-operating characteristic（ROC） curves were used to determine the diagnostic value of plasma TRAF3 in Crohn′s disease and in ulcerative colitis.Pearson correlation coefficient was used to analyze the correlation between plasma TRAF3 and endoscopic disease activity.Data were analyzed using GraphPad Prism 5.Results Plasma levels of TRAF3 were significantly higher in Crohn′s disease（P=0.000）and ulcerative colitis（P=0.000）than in healthy controls.Moreover,TRAF3 showed significant diagnostic value in distinguishing patients with Crohn′s disease（P=0.000） and ulcerative colitis（P=0.000）from healthy controls.However,no significant correlation was indicated between plasma TRAF3 and endoscopic disease activity in patients with Crohn′s disease（r=-0.093,P=0.473） or in patients with ulcerative colitis（r=0.043,P=0.733）.Conclusions Plasma levels of TRAF3 were increased in patients with inflammatory bowel disease,which showed diagnostic value in distinguishing patients with inflammatory bowel disease and healthy controls.However,plasma levels of TRAF3 could not reflect endoscopic disease activity.
出处
《现代消化及介入诊疗》
2010年第4期195-198,共4页
Modern Interventional Diagnosis and Treatment in Gastroenterology
基金
仁济医院学科建设(RJ4101305)
