摘要
为了提高猪孤雌囊胚贴壁率,试验从饲养层及培养液两方面研究猪孤雌囊胚贴壁能力;用小鼠、猪和牛的胎儿成纤维细胞制作饲养层,分别添加DMEM、NCSU-23、DMEM/NCSU-23培养液,探讨猪孤雌囊胚在3种饲养层上的发育效果。结果表明,BEF饲养层能更好地促进猪孤雌囊胚贴壁生长,其囊胚贴壁率为33.67%,与MEF饲养层组的囊胚贴壁率(19.08%)之间差异显著(P<0.05),与PEF饲养层组之间囊胚贴壁率差异不显著(P>0.05),MEF饲养层组和PEF饲养层组之间囊胚贴壁率差异不显著(P>0.05);在BEF牛胎儿成纤维细胞饲养层组,用猪胚胎培养液NCSU-23培养猪孤雌囊胚后,囊胚贴壁率(22.53%)显著高于DMEM培养液组(10.41%)和DMEM/NCSU-23培养液半量混合组(12.05%)(P<0.05),DMEM培养液组和DMEM/NCSU-23培养液半量混合组之间差异不显著(P>0.05)。牛胎儿成纤维细胞饲养层和猪胚胎培养液NCSU-23能更好地促进猪孤雌囊胚后期贴壁。
In order to improve pig parthenogenetic blastocyst adhesive rate, the experiment researchs both the culture medium and the feeder layer which can affect porcine embryo adhesive ability. Mouse, porcine and bovine fetal fibroblasts were adopted as feeder, to explore the development of the porcine parthenogenetic blastocysts cultured in DMEM or NCSU-23 or DMEM/ NCSU-23 medium. Bovine fetal fibroblasts feeder could betterly promote the porcine parthenogenetic blastocysts adherent growth, there were significant differences between the adhesion rate of bovine fetal fibroblasts feeder （33.67%） and that of mouse fetal fibroblasts feeder （19.08%）（P0.05） , while was no significant difference between the adhesion rate of bovine fetal fibroblasts feeder and that of porcine fetal fibroblasts feeder （23.47%）, the adhesion rate of mouse embryonic fibroblasts feeder group and that of the porcine fetal fibroblasts feeder groups （P0.05）. In bovine fetal fibroblasts feeder group, the blastocyst adhesion rate （22.53%） cultured in the porcine embryo culture medium NCSU-23 was significantly higher than in the DMEM culture medium （10.41%） and DMEM/NCSU-23 （12.05%） half mixed culture medium （P0.05）, and there was no significant difference between cultured in DMEM culture medium group and DMEM/NCSU-23 half mixed culture medium group （P0.05）. Bovine fetal fibroblasts feeder and porcine embryo culture medium NCSU-23 could better promote development of porcine parthenogenetic blastocyst.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第8期87-91,共5页
China Animal Husbandry & Veterinary Medicine
基金
国家"863"重点项目(2008AA101003)
关键词
猪
囊胚
饲养层
培养基
贴壁率
porcine
embryo
feeder
culture medium
adhesive rate
