摘要
草鱼干扰素 (IFN)诱生条件与规律的研究结果显示 ,病毒侵染草鱼后能迅速诱导IFN的合成 ,2 4h即可在血清中检测到IFN活性 ,3d后活性达到高峰。草鱼IFN的合成受温度、病毒种类、病毒剂量、鱼体营养状况等因素的影响。 2 5℃水温下诱生的IFN活性明显高于 15℃和 8℃。RNA病毒的诱生能力高于DNA病毒 ,其中以双链RNA病毒GCHV的诱生能力最强。在急性致死剂量以下提高病毒感染剂量以及维持鱼体的正常营养状况 ,有利于提高IFN的产量。用适量IFN或灭活GCHV预注射草鱼 ,作起动或预诱导处理 ,能显著促进IFN的诱生。病毒活性、鱼龄大小对IFN的诱生无显著影响。
In the present paper, the conditions for the induction of interferon from grass carp,Ctenopharyngodon idellus were described. The results showed that the interferon of grass carp could be induced rapidly following experimental infection with the viruses. After the virus being inoculated for 24 hours, the interferon activity in serum sample exhibited obviously. Maximum yields of serum interferon were observed after the virus being inoculated for 3 days. The production of grass carp interferon was influenced by the environmental temperature, different viral inducers, virus dosages as well as the nutrient conditions of the fish. The induction of grass carp interferon was shown to be temperature dependant. Titers of interferon induced at 25℃ was higher than that at 15℃ and 8℃. Among seven viral inducers, the interferon inducing ability of six RNA viruses was higher than that of DNA virus. And the GCHV which was of the genome of dsRNA possessed the highest interferon inducing ability. High virus dosages and good nutrient conditions for the fish were advantageous to the production of interferon. The production of grass carp interferon could also be enhanced by priming the fish with interferon or pre inducing the fish with inactivated GCHV for appropriate time. However, the influence of virus infectious activity and the ages of fish on the induction of interferon had not been observed.
出处
《水产学报》
CAS
CSCD
北大核心
1999年第2期122-127,共6页
Journal of Fisheries of China
基金
国家自然科学基金资助项目!(草鱼非特异性免疫因子--干扰素的研究 ) 3 94 0 0 10 0号
浙江省自然科学基金资助项目!(草鱼干扰素的免
关键词
草鱼
干扰素
诱生条件
免疫调节
Ctenopharyngodon idellus, Interferon, Condition for inducting
