摘要
目的:评价鲍恩样丘疹病(Bowenoid populosis,BP)皮损中p21和CDK2的表达与HPV感染的相关性。方法:石蜡包埋标本免疫组化Envision法和IMS细胞图像分析系统分析p21和CDK2蛋白表达。冰冻标本采用煮沸法提取DNA,用导流杂交基因芯片技术作HPV分型检测。结果:在28例BP中p21和CDK2阳性细胞数百分比分别为(31.6±10.5)%和(49.9±13.8)%,显著高于包皮对照组的(22.5±5.4)%和(17.3±8.5)%,P<0.05。HPV阳性组p21和CDK2阳性细胞百分比分别为(30.56±11.78)%和(54.42±13.69)%;HPV阴性组p21和CDK2阳性细胞百分比为(33.3±8.36)%和(43.15±11.55)%。p21两组差别无统计学意义(P>0.05),而CDK2差别有统计学意义(P<0.05)。p21、CDK2直线相关分析无相关性(P>0.05)。结论:BP中存在细胞周期相关蛋白的高表达,HPV可能通过作用于细胞周期相关蛋白使细胞周期紊乱,继而导致非典型增生。
Objective： To correlate the expressions of p21 and CDK2 and the infection of human papillomavirus （HPV） in Bewenoid papulosis （BP）. Methods： In the paraffin imbedded specimens, Envision immunohistology technology with image analysis system was applied to detect the expressions of p21 and CDK2. Result： In the 28 specimens of BP, the percentage of p21 and CDK2 positive cells was 31.6% ± 10.5% and 49.9% ± 13.8%, while the percentage of p21 and CDK2 positive cells was 22.5% ±5.4% and 17.3% ± 8.5% in controlled prepuce tissues. The difference was statistically significant （ P 〈 0.05）. The percentage of p21 and CDK2 positive cells was 30.56% ± 11.78% and 54.42% ± 13.69% respectively in HPV positive group. The percentage of t）21 and CDK2 positive cells was 33.3% ± 8.36% and 43.15% ± 11.55% in HPV negative group. Between the two groups, the difference of p21 was not significant （ P 〉 0.05 ）, but the difference of CDK2 was significant （ P 〈 0.05）. The expression of p21 was not correlated to CDK2 （ P 〉 0.05）. Conclusion： The expression of p21 and CDK2 are elevated in BP, suggesting the derangement of cell cycle, resulting in atypical hyperplasia.
出处
《中国麻风皮肤病杂志》
2010年第8期541-544,共4页
China Journal of Leprosy and Skin Diseases
